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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • SF3B4 Antibodies

          Invitrogen

          SF3B4 Polyclonal Antibody

          View all (11) SF3B4 antibodies

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          Cite SF3B4 Polyclonal Antibody

          • Antibody Testing Data (2)
          SF3B4 Antibody in Immunocytochemistry (ICC/IF)
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          SF3B4 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          SF3B4 Antibody (PA5-111585) in ICC/IF

          Immunofluorecent analysis of SF3B4 in human cell line MCF7 using SF3B4 Polyclonal Antibody (Product # PA5-111585). Staining shows localization to nuclear speckles. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SF3B4 Antibody in Immunocytochemistry (ICC/IF)
          SF3B4 Antibody in Western Blot (WB)
          SF3B4 Polyclonal Antibody

          Product Details

          PA5-111585

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.04-0.4 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          0.25-2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant Protein corresonding to Human SF3B4. Recombinant protein control fragment (Product #RP-105328).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.05 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 40% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2856995

          Product Specific Information

          Immunogen sequence: VSYAFKKDSK GERHGSAAER LLAAQNPLSQ ADRPHQLFAD APPPPSAPNP

          Highest antigen sequence indentity to the following orthologs: Mouse - 100%, Rat - 100%.

          Target Information

          This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: MGC10828; Pre-mRNA-splicing factor SF3b 49 kDa subunit; SAP 49; SAP49MGC10828; SF3b50; spliceosomal protein; spliceosome-associated protein (U2 snRNP); Spliceosome-associated protein 49; Splicing factor 3B subunit 4; splicing factor 3b, subunit 4, 49kD; splicing factor 3b, subunit 4, 49kDa

          View more View less

          Gene Aliases: AFD1; Hsh49; SAP49; SF3B4; SF3b49

          View more View less

          UniProt ID: (Human) Q15427

          View more View less

          Entrez Gene ID: (Human) 10262

          View more View less

          Function(s)
          nucleotide binding protein binding poly(A) RNA binding RNA splicing factor
          Process(es)
          RNA splicing, via transesterification reactions mRNA splicing, via spliceosome mRNA processing RNA splicing positive regulation of mRNA splicing, via spliceosome
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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