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Invitrogen
NLRP3 Polyclonal Antibody
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FIGURE: 1 / 5
NLRP3 Antibody (PA5-18118) in ICC/IF
Immunocytochemistry analysis of NLRP3 using NLRP3 Polyclonal Antibody (Product # PA5-18118) in paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-18118
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (EKPELTVVFEPSW) corresponding to the C-terminus amino acids of NLRP3 (aa 1024-1036).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.
This antibody is not recommended for WB.
Target Information
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site domain, and a leucine-rich repeat motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome, and neonatal-onset multisystem inflammatory disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1; CLR1.1; cold auto-inflammatory syndrome 1; PYPAF1; NALP3; C1ORF7; cold autoinflammatory syndrome 1 protein; Cold-induced autoinflammatory syndrome 1 protein; Cryopyrin; cryopyrin, NACHT, LRR and PYD domains - containing protein 3; deafness, autosomal dominant 34; FLJ95925; member of the NALP family of inflammatory mediators; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; PYD, NACHT, and LRR containing protein; PYRIN-containing APAF1-like protein 1; unnamed protein product
Gene Aliases: AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; DFNA34; FCAS; FCAS1; FCU; KEFH; MWS; NALP3; NLRP3; PYPAF1
UniProt ID: (Human) Q96P20
Entrez Gene ID: (Human) 114548
nucleotide binding
protein binding
ATP binding
hydrolase activity
ATPase activity
protein binding, bridging
signaling adaptor activity
identical protein binding
peptidoglycan binding
ADP binding
sequence-specific DNA binding
binding, bridging
phosphatidylinositol-4-phosphate binding
DNA-binding transcription factor binding
small molecule sensor activity
cysteine-type endopeptidase activator activity
molecular condensate scaffold activity
phosphatidylinositol phosphate binding
pattern recognition receptor signaling pathway
immune system process
negative regulation of acute inflammatory response
positive regulation of type 2 immune response
regulation of transcription, DNA-templated
apoptotic process
defense response
inflammatory response
signal transduction
osmosensory signaling pathway
protein secretion
detection of biotic stimulus
response to virus
negative regulation of interleukin-1 beta production
positive regulation of interleukin-1 beta production
positive regulation of interleukin-18 production
positive regulation of interleukin-4 production
interleukin-18-mediated signaling pathway
NLRP3 inflammasome complex assembly
innate immune response
positive regulation of T-helper 2 cell differentiation
positive regulation of transcription from RNA polymerase II promoter
regulation of inflammatory response
negative regulation of inflammatory response
positive regulation of inflammatory response
positive regulation of NF-kappaB transcription factor activity
protein homooligomerization
protein maturation
pyroptosis
interleukin-1-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to virus
positive regulation of NLRP3 inflammasome complex assembly
negative regulation of NIK/NF-kappaB signaling
positive regulation of NIK/NF-kappaB signaling
positive regulation of T-helper 2 cell cytokine production
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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