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Invitrogen
Emerin Polyclonal Antibody
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FIGURE: 1 / 12
Emerin Antibody (PA5-51424) in ICC/IF
Immunofluorescence analysis of Emerin was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Emerin Polyclonal Antibody (Product # PA5-51424) at 0.2 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate ... View More
Product Details
PA5-51424
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human Emerin. Recombinant protein control fragment (Product #RP-91926).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: ASSYSFSDLN STRGDADMYD LPKKEDALLY QSKGYNDDYY EESYFTTRTY GEPESAGPSR AVRQSVTSFP DADAFHHQVH DDDLLSSSEE ECKDRERPMY GRDSACQSIT HY
Highest antigen sequence identity to the following orthologs: Mouse - 71%, Rat - 73%.
Target Information
Xeroderma pigmentosum type G (XPG) is a human genetic disease exhibiting extreme sensitivity to sunlight. The XPG protein, a member of the flap endonuclease 1 (FEN-1) structure-specific DNA repair endonuclease family, is an enzyme essential for DNA repair of the major kinds of solar ultraviolet (UV)-induced DNA damages. Human XPG nuclease makes the 3' incision during nucleotide excision repair of DNA. The enzyme cleaves model DNA bubble structures specifically near the junction of unpaired DNA with a duplex region. A 29-amino acid region of human XPG (residues 981-1009) contains the PCNA binding activity. A conserved arginine in XPG (Arg992) is crucial for its PCNA binding activity. Replication Protein A (RPA) binds specifically and directly to two excision repair proteins, the xeroderma pigmentosum damage-recognition protein XPA and the endonuclease XPG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Emerin; LEM domain containing 5
Gene Aliases: AW550900; EDMD; EMD; LEMD5; STA
UniProt ID: (Human) P50402, (Mouse) O08579, (Rat) Q63190
Entrez Gene ID: (Human) 2010, (Mouse) 13726, (Rat) 25437
muscle contraction
mitotic nuclear envelope disassembly
mitotic nuclear envelope reassembly
muscle organ development
negative regulation of catenin import into nucleus
skeletal muscle cell differentiation
positive regulation of protein export from nucleus
negative regulation of fibroblast proliferation
regulation of canonical Wnt signaling pathway
cellular response to growth factor stimulus
cell-cell adhesion
nuclear envelope reassembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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