Immunofluorescent staining of IFI16 in human cell line U-251 MG using an IFI16 Polyclonal Antibody (Product # PA5-51690) shows localization to nucleus and nucleoli.

IFI16 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IFI16 Antibody in Immunohistochemistry (IHC)

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IFI16 Antibody — Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of IFI16 was observed specifically in SK-MEL-30 cells and in Caco-2 cells using anti-IFI16 Polyclonal Antibody (Product # PA5-51690) in western blot. The relative expression levels of IFI16 within each cell line is shown using RNA-Seq. {RE}

Immunogen image

Immunogen Image IFI16 Polyclonal Antibody

Product Details

PA5-51690

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human IFI16. Recombinant protein control fragment (Product #RP-101035).

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2642634

Product Specific Information

Immunogen sequence: KEKFTPKKII AIANYVCRNG FLEVYPFTLV ADVNADRNME IPKGLIRSAS VTPKINQLCS QTKGSFVNGV FEVHKKNVRG EFTYYEIQDN TGKMEVVVHG RLTTINCEEG DKLKLTCFEL APKSGNTGEL RSVIHSHIKV IK

Highest antigen sequence identity to the following orthologs: Mouse - 53%, Rat - 39%.

Target Information

IFI16 (gamma-inteferon-inducible protein 16) binds double-stranded DNA. It binds preferentially to supercoiled DNA and cruciform DNA structures. It seems to be involved in transcriptional regulation and as a transcriptional repressor. IFI16 may have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. It controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. IFI16 is also involved in innate immune response by recognizing viral dsDNA in the cytosol and nucleus. After binding to viral DNA in the cytoplasm, IFI16 recruits TMEM173/STING and mediates the induction of IFN-beta. It is necessary to activate the IFR3 signaling cascade suring human herpes simplex virus 1 infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibiton of viral immediate-early gene expression and replication. IFI16 is also involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Gamma-interferon-inducible protein 16; IFI 16C; Ifi-16; interferon, gamma-inducible protein 16; interferon-gamma induced protein IFI 16; Interferon-inducible myeloid differentiation transcriptional activator

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Gene Aliases: IFI16; IFNGIP1; PYHIN2

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UniProt ID: (Human) Q16666

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Entrez Gene ID: (Human) 3428

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding double-stranded DNA binding protein binding transcription factor binding poly(A) RNA binding DNA-binding transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter positive regulation of cytokine production activation of innate immune response transcription, DNA-templated autophagy inflammatory response cell proliferation regulation of autophagy hemopoiesis myeloid cell differentiation monocyte differentiation positive regulation of type I interferon production positive regulation of interleukin-1 beta production regulation of gene expression, epigenetic cellular response to glucose starvation intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of DNA binding negative regulation of viral genome replication innate immune response negative regulation of innate immune response negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter defense response to virus cellular response to ionizing radiation intrinsic apoptotic signaling pathway by p53 class mediator activation of cysteine-type endopeptidase activity negative regulation of cysteine-type endopeptidase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IFI16 Polyclonal Antibody

IFI16 Antibody (PA5-51690) in ICC/IF

Product Details

PA5-51690

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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