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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • SEC31A Antibodies

          Invitrogen

          SEC31A Polyclonal Antibody

          Advanced Verification
          View all (8) SEC31A antibodies

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          Cite SEC31A Polyclonal Antibody

          • Antibody Testing Data (8)
          • Advanced Verification (1)
          SEC31A Antibody in Immunocytochemistry (ICC/IF)
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          SEC31A Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          SEC31A Antibody (PA5-52147) in ICC/IF

          Immunofluorescence analysis of SEC31A was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SEC31A Polyclonal Antibody (Product # PA5-52147) at 2 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilu... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          SEC31A Antibody in Immunocytochemistry (ICC/IF)
          SEC31A Antibody in Immunocytochemistry (ICC/IF)
          SEC31A Antibody in Immunohistochemistry (IHC)
          SEC31A Antibody in Immunohistochemistry (IHC)
          SEC31A Antibody in Immunohistochemistry (IHC)
          SEC31A Antibody in Immunohistochemistry (IHC)
          SEC31A Antibody in Western Blot (WB)
          SEC31A Antibody in Western Blot (WB)
          SEC31A Antibody
          SEC31A Polyclonal Antibody

          Product Details

          PA5-52147

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.4 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          0.25-2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein corresponding to Human SEC31A. Recombinant protein control fragment (Product #RP-89208).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 40% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2647080

          Product Specific Information

          Immunogen sequence: NWREALAAVL TYAKPDEFSA LCDLLGTRLE NEGDSLLQTQ ACLCYICAGN VEKLVACWTK AQDGSHPLSL QDLIEKVVIL RKAVQLTQAM DTSTVGVLLA AKMSQYANLL AAQGSIAAAL AFLPDNTNQP NIMQLR

          Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 92%.

          Target Information

          The protein encoded by this gene is similar to yeast Sec31 protein. Yeast Sec31 protein is known to be a component of the COPII protein complex which is responsible for vesicle budding from endoplasmic reticulum (ER). This protein was found to colocalize with SEC13, one of the other components of COPII, in the subcellular structures corresponding to the vesicle transport function. An immunodepletion experiment confirmed that this protein is required for ER-Golgi transport. Alternative splicing results in multiple transcript variants encoding different isoforms.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: ABP125; ABP130; Protein transport protein Sec31A; SEC31 homolog A, COPII coating complex component; SEC31-like protein 1; SEC31-related protein A; Web1-like protein; yeast Sec31p homolog

          View more View less

          Gene Aliases: ABP125; ABP130; HSPC275; HSPC334; KIAA0905; SEC31A; SEC31L1

          View more View less

          UniProt ID: (Human) O94979

          View more View less

          Entrez Gene ID: (Human) 22872

          View more View less

          Function(s)
          protein binding calcium-dependent protein binding vesicle coat protein
          Process(es)
          antigen processing and presentation of peptide antigen via MHC class I ER to Golgi vesicle-mediated transport protein transport antigen processing and presentation of exogenous peptide antigen via MHC class II IRE1-mediated unfolded protein response COPII vesicle coating response to calcium ion
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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