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Invitrogen
DHX9 Polyclonal Antibody
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FIGURE: 1 / 13
DHX9 Antibody (PA5-55754) in ICC/IF
Immunofluorescence analysis of DHX9 was performed using A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DHX9 Polyclonal Antibody (Product # PA5-55754) at 4 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:200... View More
Product Details
PA5-55754
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human DHX9. Recombinant protein control fragment (Product #RP-94224).
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: PHLALKAENN SEVGASGYGV PGPTWDRGAN LKDYYSRKEE QEVQATLESE EVDLNAGLHG NWTLENAKAR LNQYFQKEKI QGEYKYTQVG PDHNRSF
Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 94%.
Target Information
The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATP-dependent RNA helicase A; D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 9; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 9; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9; DEAH (Asp-Glu-Ala-His) box helicase 9; DEAH (Asp-Glu-Ala-His) box polypeptide 9; DEAH box protein 9; DExH-box helicase 9; FLJ17406; Leukophysin; LKP; mHEL-5; NDH II; Nuclear DNA helicase II; OTTHUMP00000033287; RHA; RNA helicase; RNA helicase A
Gene Aliases: AI326842; DDX9; DHX9; HEL-5; LKP; mHEL-5; NDH2; NDHII; RHA
UniProt ID: (Human) Q08211, (Mouse) O70133
Entrez Gene ID: (Human) 1660, (Mouse) 13211, (Rat) 304859
RNA polymerase II transcription factor binding
DNA binding
RNA helicase activity
ATP-dependent DNA helicase activity
ATP-dependent RNA helicase activity
protein binding
ATP binding
poly(A) RNA binding
nucleotide binding
nucleic acid binding
RNA binding
helicase activity
ATP-dependent helicase activity
hydrolase activity
molecular_function
RNA helicase
mRNA splicing, via spliceosome
osteoblast differentiation
RNA processing
positive regulation of type I interferon production
DNA duplex unwinding
rhythmic process
CRD-mediated mRNA stabilization
protein localization to cytoplasmic stress granule
circadian rhythm
cellular response to heat
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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