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          • Primary Antibodies ›
          • STAG1 Antibodies

          Invitrogen

          STAG1 Polyclonal Antibody

          View all (15) STAG1 antibodies

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          Cite STAG1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (10)
          STAG1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          STAG1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 10

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          STAG1 Antibody (PA5-57115) in ICC/IF

          Immunofluorescence analysis of Cohesin subunit SA-1 was performed using 70% confluent log phase MCF 10A cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with STAG1 Polyclonal Antibody (Product # PA5-57115) at 0.5 ug/ml in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Produ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          STAG1 Antibody in Immunocytochemistry (ICC/IF)
          STAG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          STAG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          STAG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          STAG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          STAG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          STAG1 Antibody in Western Blot (WB)
          STAG1 Antibody in Western Blot (WB)
          STAG1 Antibody in Western Blot (WB)
          STAG1 Antibody in Western Blot (WB)
          STAG1 Polyclonal Antibody

          Product Details

          PA5-57115

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.04-0.4 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          0.25-2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein corresponding to Human STAG1. Recombinant protein control fragment (Product #RP-96325).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.1 mg/mL

          Amount

          10 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 40% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2647987

          Product Specific Information

          Immunogen sequence: TSELPVLQDS TNETTAHSDA GSELEETEVK GKRKRGRPGR PPSTNKKPRK SPGEKSR

          Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.

          Target Information

          This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Cohesin subunit SA-1; DKFZp781D1416; nuclear protein stromal antigen 1; SCC3 homolog 1; sister chromatid cohesion 3 homolog A; Stromal antigen 1; unnamed protein product

          View more View less

          Gene Aliases: AU045003; MRD47; SA-1; SA1; SCC3; SCC3A; STAG1

          View more View less

          UniProt ID: (Human) Q8WVM7, (Mouse) Q9D3E6

          View more View less

          Entrez Gene ID: (Human) 10274, (Mouse) 20842, (Rat) 315958

          View more View less

          Function(s)
          chromatin binding protein binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin/chromatin-binding, or -regulatory protein
          Process(es)
          chromosome segregation sister chromatid cohesion establishment of mitotic sister chromatid cohesion cell division mitotic spindle assembly transcription from RNA polymerase II promoter cell cycle mitotic nuclear division positive regulation of transcription from RNA polymerase II promoter
          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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