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Invitrogen
OPA1 Polyclonal Antibody
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FIGURE: 1 / 9
OPA1 Antibody (PA5-57874) in ICC/IF
Immunofluorescence analysis of OPA1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with OPA1 Polyclonal Antibody (Product # PA5-57874) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790... View More
Product Details
PA5-57874
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human OPA1. Recombinant protein control fragment (Product #RP-96480).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: ESIKRHKWND FAEDSLRVIQ HNALEDRSIS DKQQWDAAIY FMEEALQARL KDTENAIENM VGPDWKKRWL YWKNRTQEQC VHNETKNELE KMLKCNE
Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 95%.
Target Information
OPA1 is a dynamin-related GTPase that is critical for the maintenance of mitochondrial morphology and mtDNA. The most commonly associated phenotype with OPA1 mutations is heterozygous optic atrophy, a heterozygous dominant trait that causes reduced visual clarity and sometimes blindness. The disease usually begins in childhood and increases in severity throughout the life of affected individuals. Usually, this phenotype is attributed to the degeneration of optic nerve fibers. Interestingly, the same type of nerve degeneration seems to be partially causative of certain schizophrenia characteristics. OPA1 dysfunction also seems to be implicated in this case; mitochondrial networks associated with critical nerves seem to link schizophrenia and OPA1. The dysfunction is associated with issues with apoptosis and normal cellular metabolic regulation, all regulated through OPA1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dynamin-like 120 kDa protein, mitochondrial; Dynamin-like GTPase OPA1, mitochondrial; dynamin-like guanosine triphosphatase; mitochondrial dynamin-like GTPase; optic atrophy 1 (autosomal dominant); Optic atrophy protein 1
Gene Aliases: BERHS; KIAA0567; largeG; MGM1; MTDPS14; NPG; NTG; OPA1
UniProt ID: (Human) O60313
Entrez Gene ID: (Human) 4976
mitochondrial genome maintenance
mitochondrial fission
neural tube closure
apoptotic process
mitochondrion organization
inner mitochondrial membrane organization
visual perception
mitochondrial fusion
regulation of mitochondrion organization
peroxisome fission
axonal transport of mitochondrion
positive regulation of interleukin-17 production
cristae formation
negative regulation of apoptotic process
GTP metabolic process
protein oligomerization
membrane fusion
negative regulation of release of cytochrome c from mitochondria
cellular senescence
membrane tubulation
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
mitochondrial inner membrane fusion
positive regulation of T-helper 17 cell lineage commitment
negative regulation of intrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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