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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • NIT2 Antibodies

          Invitrogen

          NIT2 Polyclonal Antibody

          View all (27) NIT2 antibodies

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          Cite NIT2 Polyclonal Antibody

          • Antibody Testing Data (4)
          NIT2 Antibody in Immunocytochemistry (ICC/IF)
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          NIT2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          NIT2 Antibody (PA5-57908) in ICC/IF

          Immunofluorecent analysis of NIT2 in human cell line U-251 MG using NIT2 Polyclonal Antibody (Product # PA5-57908). Staining shows localization to cytosol and centrosome. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          NIT2 Antibody in Immunocytochemistry (ICC/IF)
          NIT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          NIT2 Antibody in Western Blot (WB)
          NIT2 Antibody in Western Blot (WB)
          NIT2 Polyclonal Antibody

          Product Details

          PA5-57908

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.04-0.4 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200-1:500
          -

          Immunocytochemistry (ICC/IF)

          0.25-2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein corresponding to Human NIT2. Recombinant protein control fragment (Product #RP-96272).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 40% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2644694

          Product Specific Information

          Immunogen sequence: YLIGGSIPEE DAGKLYNTCA VFGPDGTLLA KYRKIHLFDI DVPGKITFQE SKTLSPGDSF STFDTPYCRV GLGICYDMRF AELAQIYAQ

          Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 94%.

          Target Information

          Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

          Have you cited this product in a publication?
          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: epididymis secretory sperm binding protein Li 8a; Nit protein 2; nitrilase family, member 2; Nitrilase homolog 2; Omega-amidase NIT2

          View more View less

          Gene Aliases: 1190017B19Rik; CUA002; D16Ertd502e; HEL-S-8a; NIT2; RGD1310494

          View more View less

          UniProt ID: (Human) Q9NQR4, (Rat) Q497B0, (Mouse) Q9JHW2

          View more View less

          Entrez Gene ID: (Human) 56954, (Rat) 288174, (Mouse) 52633

          View more View less

          Function(s)
          omega-amidase activity hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds hydrolase
          Process(es)
          oxaloacetate metabolic process asparagine metabolic process glutamine metabolic process nitrogen compound metabolic process
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
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          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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