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Invitrogen
AFAP1L2 Polyclonal Antibody
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Product Details
PA5-58134
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human AFAP1L2. Recombinant protein control fragment (Product #RP-97174).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: SPTLSCLDNA TEALPADSGP GPTPDEPCIK CPENLGEQQL ESLEPEDPSL RITTVKIQTE QQRISFPPSC PDAVVATPPG ASPPVKD
Highest antigen sequence identity to the following orthologs: Mouse - 47%, Rat - 49%.
Target Information
AFAP1L2, also known as XB130, is structurally similar to actin-filament-associated protein (AFAP), containing several SH2- and SH3-binding motifs, two pleckstrin homology domains, a coiled-coil region, and many potential phosphorylation sites. It interacts with and is phosphorylated by c-Src tyrosine kinase. Suppression of AFAP1L2 via siRNA reduced Src activity, IL-8 production, EGF-induced phosphorylation of Akt and GSK3beta, and altered cell cycles in human lung epithelial cells suggesting that AFAP1L2 plays a role as an adaptor in the regulation of Src signal transduction and multiple cellular functions. Recent experiments have shown that AFAP1L2 is highly expressed in thyroid and is the substrate RET/PTC kinase, a thyroid-specific kinase that plays a pathogenic role in papillary thyroid cancer. Down-regulation of AFAP1L2 in these cancer cells reduced Akt activity, inhibiting cell-cycle progression and cancer cell survival in suspension, indicating that AFAP1L2 may be a valuable target in thyroid cancer therapy. At least four isoforms of AFAP1L2 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: actin filament associated protein 1-like 2; Actin filament-associated protein 1-like 2; AFAP1-like protein 2; CTB-1144G6.6
Gene Aliases: AFAP1L2; CTB-1144G6.4; KIAA1914; XB130
UniProt ID: (Human) Q8N4X5
Entrez Gene ID: (Human) 84632
inflammatory response
regulation of mitotic cell cycle
regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of epidermal growth factor receptor signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of protein tyrosine kinase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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