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Invitrogen
HIC1 Polyclonal Antibody
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Product Details
PA5-60343
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human HIC1. Recombinant protein control fragment (Product #RP-99937).
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Amount
5 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: ELLAQTTHFL HDPKVALESL YPLAKFTAEL GLSPDKAAEV LSQGAHLAAG PDGRTIDRFS PT
Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 97%.
Target Information
Hypermethylated in cancer (HIC-1) was originally identified as a target of p53-induced gene expression. HIC-1 is deleted in the genetic disorder Miller-Dieker syndrome (MDS), and the expression of HIC-1 is also frequently suppressed in leukemia and various cancers due to the hypermethylation of specific DNA regions and the resulting transcriptional silencing. These and other studies indicate that HIC-1 acts as a putative tumor suppressor protein that mediates transcriptional repression. HIC-1 is ubiquitously expressed in adult tissues and its structure is defined by five zinc fingers and an N-terminal broad complex POZ (or BTB) domain. In several BTB/POZ containing proteins, including BCL-6 and the promyelocytic leukemia zinc-finger (PLZF) oncoprotein, this domain interacts with the SMRT/N-CoR-mSin3A HDAC complex and is directly involved in repressing and silencing gene transcription. When this domain is deleted, as with the oncogenic PLZF-RAR chimera of promyelocytic leukemias, this transcriptional repression is attenuated. Conversely, HIC-1 does not interact with components of the HDAC complex, suggesting that HIC-1-induced transcriptional repression is unassociated with the POZ/BTB domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hic-1; hypermethylated in cancer 1; Hypermethylated in cancer 1 protein; hypermethylated in cancer-1; Zinc finger and BTB domain-containing protein 29
Gene Aliases: hic-1; HIC1; ZBTB29; ZNF901
UniProt ID: (Human) Q14526
Entrez Gene ID: (Human) 3090
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
histone deacetylase binding
sequence-specific DNA binding
metal ion binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
intrinsic apoptotic signaling pathway in response to DNA damage
regulation of signal transduction
Wnt signaling pathway
negative regulation of Wnt signaling pathway
positive regulation of DNA damage response, signal transduction by p53 class mediator
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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