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Invitrogen
TRIP12 Polyclonal Antibody
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Product Details
PA5-61116
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human TRIP12. Recombinant protein control fragment (Product #RP-96152).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LNSGNVRHRL QFYIGEHLLP YNMTVYQAVR QFSIQAEDER ESTDDESNPL GRAGIWTKTH TIWYKPVRED EESNKDCVGG KRGRAQTAPT KTSP
Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 98%.
Target Information
E3 ubiquitin-protein ligase involved in ubiquitin fusion degradation (UFD) pathway and regulation of DNA repair. Part of the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination of protein at their N-terminus, regardeless of the presence of lysine residues in target proteins. In normal cells, mediates ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A, a lysine-less tumor suppressor required for p53/TP53 activation under oncogenic stress. In cancer cells, however, isoform p19ARF/ARF and TRIP12 are located in different cell compartments, preventing isoform p19ARF/ARF ubiquitination and degradation. Does not mediate ubiquitination of isoform p16-INK4a of CDKN2A. Also catalyzes ubiquitination of NAE1 and SMARCE1, leading to their degradation. Ubiquitination and degradation of target proteins is regulated by interaction with proteins such as MYC, TRADD or SMARCC1, which disrupt the interaction between TRIP12 and target proteins. Acts as a key regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase for Arf; E3 ubiquitin-protein ligase TRIP12; HECT-type E3 ubiquitin transferase TRIP12; KIAA0045; probable E3 ubiquitin-protein ligase TRIP12; specific ubiquitin ligase for ARF; thyroid receptor interacting protein 12; Thyroid receptor-interacting protein 12; TR-interacting protein 12; ULF; unnamed protein product
Gene Aliases: KIAA0045; MRD49; TRIP-12; TRIP12; TRIPC; ULF
UniProt ID: (Human) Q14669
Entrez Gene ID: (Human) 9320
protein polyubiquitination
DNA repair
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
protein ubiquitination
negative regulation of extent of heterochromatin assembly
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of embryonic development
DNA repair-dependent chromatin remodeling
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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