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Invitrogen
NUDT16 Polyclonal Antibody
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Product Details
PA5-64026
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human NUDT16. Recombinant protein control fragment (Product #RP-103452).
Conjugate
Form
Liquid
Concentration
0.3 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: SAREQLLEAL QDLGLLQSGS ISGLKIPAHH
Highest antigen sequence identity to the following orthologs: Mouse - 67%, Rat - 60%.
Target Information
RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA; metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase; hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms (PubMed:20385596, PubMed:26121039). The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP (PubMed:20385596). Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions (PubMed:20385596). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: IDP phosphatase; IDPase; Inosine diphosphate phosphatase; m7GpppN-mRNA hydrolase; Nucleoside diphosphate-linked moiety X motif 16; nudix (nucleoside diphosphate linked moiety X)-type motif 16; Nudix hydrolase 16; Nudix motif 16; testicular tissue protein Li 129; U8 snoRNA-binding protein H29K; U8 snoRNA-decapping enzyme
Gene Aliases: NUDT16
UniProt ID: (Human) Q96DE0
Entrez Gene ID: (Human) 131870
magnesium ion binding
mRNA binding
GTP binding
metalloexopeptidase activity
manganese ion binding
snoRNA binding
dITP diphosphatase activity
identical protein binding
protein homodimerization activity
m7G(5')pppN diphosphatase activity
cobalt ion binding
inosine-diphosphatase activity
dIDP diphosphatase activity
nucleotide phosphatase activity, acting on free nucleotides
XTP binding
ITP binding
adenosine to inosine editing
mRNA catabolic process
proteolysis
positive regulation of cell proliferation
snoRNA catabolic process
dephosphorylation
nucleobase-containing small molecule catabolic process
dITP catabolic process
IDP catabolic process
positive regulation of cell cycle process
XDP catabolic process
negative regulation of rRNA processing
positive regulation of double-strand break repair
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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