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Invitrogen
BHLHB5 Polyclonal Antibody
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FIGURE: 1 / 9
BHLHB5 Antibody (PA5-64189) in ICC/IF
Immunofluorescence analysis of BHLHB5 was performed using log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with BHLHB5 Polyclonal Antibody (Product # PA5-64189) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dil... View More
Product Details
PA5-64189
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human BHLHB5. Recombinant protein control fragment (Product #RP-103577).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: EDDLFLHKSL SASTSKRLEA AFRSTPPGMD LSLAPPPRER PASSSSSPLG CFEPADPEGA GLLLPPP
Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 93%.
Target Information
BHLHB5 inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. BHLHB5 despite the presence of an intact basic domain, does not bind to DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: basic helix-loop-helix domain containing, class B, 5; basic helix-loop-helix family, member e22; bHLHb5; bHLHe22; Class B basic helix-loop-helix protein 5; Class E basic helix-loop-helix protein 22; trinucleotide repeat containing 20; Trinucleotide repeat-containing gene 20 protein
Gene Aliases: Beta3; BHLHB5; BHLHE22; CAGL85; TNRC20
UniProt ID: (Human) Q8NFJ8
Entrez Gene ID: (Human) 27319
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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