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Invitrogen
c-Raf Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-82026
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human RAF1. Recombinant protein control fragment (Product #RP-102057).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.05 mg/mL
Amount
5 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: FRCQTCGYKF HEHCSTKVPT MCVDWSNIRQ LLLFPNSTIG DSGVPALPSL TMRRMRESVS RMPVSSQHRY STPHAFTFNT SSPSSEGSLS QRQRSTSTPN VHMVSTTLPV DSRMIEDAIR SHSESASPSA LSSSPNNLSP TGWSQ
Highest antigen sequence indentity to the following orthologs: Rat - 97%, Mouse - 96%.
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cRaf; EC 2.7.11.1; kinase Raf1; OTTHUMP00000207813; OTTHUMP00000209389; Proto-oncogene c-RAF; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; vraf1 murine le
Gene Aliases: RAF; RAF1
Entrez Gene ID: (Human) 5894
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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