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Invitrogen
NeuroD1 Polyclonal Antibody
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FIGURE: 1 / 4
NeuroD1 Antibody (PA5-82075) in ICC/IF
Immunofluorescence analysis of NEUROD1 was performed using 70% confluent log phase IMR-32 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NeuroD1 Polyclonal Antibody (Product # PA5-82075) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product ... View More
Product Details
PA5-82075
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human NEUROD1. Recombinant protein control fragment (Product #RP-100735).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: ASFPVHPYSY QSPGLPSPPY GTMDSSHVFH VKPPPHAYSA ALEPFFESPL TDCTSPSFDG PLSPPLSING NFSFKHEPSA EFEKNYAFTM HYPAATLAGA QSHGSIFSGT AAPRCEIPID NIMSFDSHSH HERVMSAQL
Highest antigen sequence indentity to the following orthologs: Rat - 97%, Mouse - 98%.
Target Information
NeuroD1 is a transcriptional activator that acts as a differentiation factor during neurogenesis. It has been demonstrated to bind to the insulin gene E-box. Efficient DNA binding requires dimerization with another basic helix-loop-helix (bHLH) protein. Defects in NEUROD1 are a cause of maturity onset diabetes of the young type VI (MODY6). MODY6 is a form of non-insulin-dependent diabetes mellitus characterized by an autosomal dominant mode of inheritance, onset during young adulthood and a primary defect in insulin secretion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: basic helix-loop-helix transcription factor; beta-cell E-box transactivator 2; BHF1; bHLHa3; Class A basic helix-loop-helix protein 3; neuro d1; NeuroD; Neurogenic differentiation factor 1; neurogenic helix-loop-helix protein NEUROD; neurogenic helix-loop-helix protein NEUROD; basic helix-loop-helix transcription factor; beta-cell E-box transactivator 2; unnamed protein product
Gene Aliases: BETA2; BHF-1; BHLHA3; MODY6; NEUROD; NEUROD1; T2D
UniProt ID: (Human) Q13562
Entrez Gene ID: (Human) 4760
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
protein heterodimerization activity
protein dimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
E-box binding
sequence-specific double-stranded DNA binding
pancreatic A cell fate commitment
pancreatic PP cell fate commitment
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
JAK-STAT cascade
nervous system development
sensory organ development
response to glucose
anterior/posterior pattern specification
dentate gyrus development
cerebellum development
neurogenesis
signal transduction involved in regulation of gene expression
insulin secretion
cell differentiation
hindbrain development
endocrine pancreas development
positive regulation of insulin secretion
amacrine cell differentiation
enteroendocrine cell differentiation
glucose homeostasis
camera-type eye development
positive regulation of apoptotic process
cell fate commitment
positive regulation of cell differentiation
regulation of neuron differentiation
positive regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of JAK-STAT cascade
embryonic organ morphogenesis
inner ear development
regulation of insulin secretion
positive regulation of sequence-specific DNA binding transcription factor activity
regulation of intestinal epithelial structure maintenance
axon development
cellular response to glucocorticoid stimulus
negative regulation of type B pancreatic cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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