Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
alpha Actinin 1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 7
alpha Actinin 1 Antibody (PA5-82233) in ICC/IF
Immunofluorescence analysis of alpha Actinin 1 was performed using 70 confluent log phase HSkMs cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with alpha Actinin 1 Polyclonal Antibody (Product # PA5-82233, 1:100) in 0.1% BSA, incubated at 4 degree cel... View More
Product Details
PA5-82233
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human ACTN1. Recombinant protein control fragment (Product #RP-88918).
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: SAKEGLLLWC QRKTAPYKNV NIQNFHISWK DGLGFCALIH RHRPELIDYG KLRKDDPLTN LNTAFDVAEK YLDIPKMLDA EDIVGTARPD EKAIMTYVSS FYHAFSGAQK AETAANRICK V
Highest antigen sequence indentity to the following orthologs: Mouse - 98%, Rat - 99%.
Target Information
Alpha Actinin 1 belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: actinin 1 smooth muscle; actinin, alpha 1; alpha actinin 1a; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; F-actin cross-linking protein; FLJ40884; FLJ54432; non-muscle alpha-actinin 1; Non-muscle alpha-actinin-1
Gene Aliases: 3110023F10Rik; ACTN1; Actn1a; BDPLT15
UniProt ID: (Human) P12814, (Rat) Q9Z1P2, (Mouse) Q7TPR4
Entrez Gene ID: (Human) 87, (Rat) 81634, (Mouse) 109711
double-stranded RNA binding
integrin binding
calcium ion binding
protein binding
vinculin binding
ligand-dependent nuclear receptor transcription coactivator activity
protein homodimerization activity
ion channel binding
actin filament binding
protein domain specific binding
actin binding
metal ion binding
platelet degranulation
actin filament organization
platelet activation
platelet formation
platelet morphogenesis
regulation of apoptotic process
focal adhesion assembly
actin filament bundle assembly
negative regulation of cellular component movement
actin filament network formation
actin crosslink formation
platelet aggregation
regulation of nucleic acid-templated transcription
cortical cytoskeleton organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support