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Invitrogen

ROCK1 Polyclonal Antibody

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ROCK1 Polyclonal Antibody

Product Details

PA5-82303

Applications
Tested Dilution
Publications

Western Blot (WB)

0.04-0.4 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ROCK1. Recombinant protein control fragment (Product #RP-102054).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2789462

Product Specific Information

Immunogen sequence: TQVKELKEEI EEKNRENLKK IQELQNEKET LATQLDLAET KAESEQLARG LLEEQYFELT QESKKAASRN RQEITDKDHT VSRLEEANSM LTKDIEILRR ENEELTEKMK KAEEEYKLEK EEEISNLKAA FEKNINTERT

Highest antigen sequence indentity to the following orthologs: Mouse - 89%, Rat - 90%.

Target Information

ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: liver regeneration-related protein LRRG199; MGC131603; MGC43611; p150 RhoA-binding kinase ROK beta; p160 ROCK-1; p160-ROCK; p160ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho kinase; Rho-associated coiled-coil forming kinase 1; Rho-associated kinase beta; Rho-associated protein kinase 1; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I

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Gene Aliases: 1110055K06Rik; P160ROCK; ROCK-I; ROCK1

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UniProt ID: (Human) Q13464, (Mouse) P70335

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Entrez Gene ID: (Human) 6093, (Mouse) 19877, (Rat) 81762

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Function(s)
nucleotide binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding zinc ion binding kinase activity transferase activity small GTPase binding metal ion binding tau protein binding tau-protein kinase activity Rho-dependent protein serine/threonine kinase activity protein serine kinase activity transferase activity, transferring phosphorus-containing groups Rho GTPase binding GTP-Rho binding non-receptor serine/threonine protein kinase
Process(es)
mitotic cytokinesis epithelial to mesenchymal transition aortic valve morphogenesis apical constriction apoptotic process smooth muscle contraction leukocyte cell-cell adhesion signal transduction Rho protein signal transduction regulation of gene expression positive regulation of autophagy positive regulation of macromolecule metabolic process positive regulation of cardiac muscle hypertrophy positive regulation of gene expression negative regulation of angiogenesis peptidyl-serine phosphorylation membrane to membrane docking actin cytoskeleton organization regulation of cell adhesion regulation of cell migration cortical actin cytoskeleton organization actomyosin structure organization neuron projection development bleb assembly negative regulation of protein binding regulation of actin cytoskeleton organization regulation of actin filament-based process intracellular signal transduction positive regulation of MAPK cascade regulation of keratinocyte differentiation regulation of neuron differentiation positive regulation of phosphate metabolic process embryonic morphogenesis leukocyte migration leukocyte tethering or rolling negative regulation of membrane protein ectodomain proteolysis myoblast migration regulation of stress fiber assembly regulation of focal adhesion assembly positive regulation of focal adhesion assembly mRNA destabilization negative regulation of biomineral tissue development regulation of microtubule cytoskeleton organization response to transforming growth factor beta protein localization to plasma membrane regulation of synapse maturation glomerular visceral epithelial cell migration motor neuron apoptotic process regulation of blood vessel diameter regulation of angiotensin-activated signaling pathway neuron projection arborization positive regulation of beta-amyloid clearance regulation of synaptic vesicle endocytosis regulation of cell junction assembly regulation of beta-amyloid formation negative regulation of beta-amyloid formation negative regulation of amyloid precursor protein catabolic process regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly positive regulation of connective tissue replacement response to angiotensin regulation of establishment of cell polarity regulation of cell motility negative regulation of motor neuron apoptotic process protein phosphorylation cytoskeleton organization phosphorylation negative regulation of neuron apoptotic process regulation of cytoskeleton organization
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