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Invitrogen
ROCK1 Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-82303
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human ROCK1. Recombinant protein control fragment (Product #RP-102054).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: TQVKELKEEI EEKNRENLKK IQELQNEKET LATQLDLAET KAESEQLARG LLEEQYFELT QESKKAASRN RQEITDKDHT VSRLEEANSM LTKDIEILRR ENEELTEKMK KAEEEYKLEK EEEISNLKAA FEKNINTERT
Highest antigen sequence indentity to the following orthologs: Mouse - 89%, Rat - 90%.
Target Information
ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: liver regeneration-related protein LRRG199; MGC131603; MGC43611; p150 RhoA-binding kinase ROK beta; p160 ROCK-1; p160-ROCK; p160ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho kinase; Rho-associated coiled-coil forming kinase 1; Rho-associated kinase beta; Rho-associated protein kinase 1; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I
Gene Aliases: 1110055K06Rik; P160Rock; ROCK-I; ROCK1
UniProt ID: (Mouse) P70335
Entrez Gene ID: (Mouse) 19877, (Rat) 81762
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Rho GTPase binding
GTP-Rho binding
metal ion binding
non-receptor serine/threonine protein kinase
apical constriction
protein phosphorylation
apoptotic process
cytoskeleton organization
leukocyte cell-cell adhesion
signal transduction
Rho protein signal transduction
phosphorylation
negative regulation of angiogenesis
membrane to membrane docking
actin cytoskeleton organization
bleb assembly
negative regulation of protein binding
regulation of actin filament-based process
intracellular signal transduction
negative regulation of neuron apoptotic process
regulation of keratinocyte differentiation
leukocyte migration
leukocyte tethering or rolling
myoblast migration
regulation of stress fiber assembly
regulation of cytoskeleton organization
positive regulation of focal adhesion assembly
regulation of establishment of cell polarity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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