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Invitrogen
CD223 Polyclonal Antibody
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FIGURE: 1 / 5
CD223 Antibody (PA5-82490) in IHC (P)
Immunohistochemical analysis of CD223 (LAG-3) was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minu... View More
Product Details
PA5-82490
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human LAG3. Recombinant protein control fragment (Product #RP-90012).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LVTGDNGDFT LRLEDVSQAQ AGTYTCHIHL QEQQLNATVT LAIITVTPKS FGSPGSLGKL LCEVTPVSGQ ERFVWSSLDT PSQRSFSGPW LEAQEAQLLS QPWQCQLYQG ERLLGAAVYF TELSSPGAQ
Highest antigen sequence indentity to the following orthologs: Mouse - 69%, Rat - 64%.
Target Information
LAG-3 is a 70-kDa surface glycoprotein in the immunoglobulin superfamily, with structural homology to CD4. It binds to MHC class II molecules with higher affinity than CD4 and is involved in the negative regulation of T cell activation and homeostatic proliferation. LAG-3 is expressed on the surface of activated T cells, including regulatory T cells, and NK cells. Notably, CD8+ T cells express LAG-3 at significantly higher levels than CD4+ T cells. Additionally, the coexpression of LAG-3 and CD49b has been proposed as a marker for identifying Type 1 regulatory T cells (Tr1 cells) in both humans and mice. This highlights LAG-3s role in immune regulation and its potential as a target for therapeutic interventions in immune-related conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD223; LAG-3; Lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; sLAG 3; sLAG3; soluble LAG 3lymphocyte activating 3; soluble LAG3; unnamed protein product
Gene Aliases: CD223; FDC; LAG3
UniProt ID: (Human) P18627
Entrez Gene ID: (Human) 3902
adaptive immune response
plasmacytoid dendritic cell activation
immune system process
cell surface receptor signaling pathway
negative regulation of interleukin-2 production
T cell activation
natural killer cell mediated cytotoxicity
innate immune response
negative regulation of regulatory T cell differentiation
positive regulation of natural killer cell mediated cytotoxicity
regulation of immune response
negative regulation of T cell activation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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