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Invitrogen
USP25 Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-82623
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human USP25. Recombinant protein control fragment (Product #RP-91812).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.4 mg/mL
Amount
40 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: QPLVGIETLP PDLRDFVEED NQRFEKELEE WDAQLAQKAL QEKLLASQKL RESETSVTTA QAAGDPEYLE QPSRSDFSKH LKEETIQIIT KASHEHEDKS PETVLQSIMM TPNMQGIIMA IGKSRSVYDR CGPEAGFFK
Highest antigen sequence indentity to the following orthologs: Rat - 62%, Mouse - 60%.
Target Information
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: deubiquitinating enzyme; deubiquitinating enzyme 25; ubiquitin thioesterase 25; ubiquitin thiolesterase 25; ubiquitin-specific processing protease 25; unnamed protein product; USP on chromosome 21; USP25m; only found in skeletal muscle and heart
Gene Aliases: EIG19; USP21
UniProt ID: (Human) Q9UHP3
Entrez Gene ID: (Human) 29761
proteolysis
immune response
response to oxidative stress
mRNA metabolic process
protein deubiquitination
stress-activated protein kinase signaling cascade
regulation of protein stability
protein modification process
protein-containing complex assembly
protein K63-linked deubiquitination
protein K48-linked deubiquitination
interleukin-17-mediated signaling pathway
negative regulation of response to oxidative stress
negative regulation of interleukin-17-mediated signaling pathway
negative regulation of ERAD pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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