Invitrogen

AP2 alpha Polyclonal Antibody

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AP2 alpha Polyclonal Antibody

Product Details

PA5-83036

Applications
Tested Dilution
Publications

Western Blot (WB)

0.04-0.4 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:2,500
-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human TFAP2A. Recombinant protein control fragment (Product #RP-94906).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.7 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2790192

Product Specific Information

Immunogen sequence: RHEDLLHGPH ALSSGLGDLS IHSLPHAIEE VPHVEDPGIN IPDQTVIKKG PVSLSKSNSN AVSAIPINKD

Highest antigen sequence indentity to the following orthologs: Mouse - 94%, Rat - 94%.

Target Information

TFAP2A is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limbs and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. TFAP2A is the only AP-2 protein required for early morphogenesis of the lens vesicle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: activating enhancer-binding protein 2 alpha; Activating enhancer-binding protein 2-alpha; Activator protein 2; AP-2; AP-2 transcription factor; AP2-alpha; ERF-1; FLJ51761; OTTHUMP00000214240; OTTHUMP00000214243; transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha); Transcription factor AP-2-alpha

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Gene Aliases: AP-2; AP-2alpha; AP2TF; BOFS; Tcfap2a; TFAP2; TFAP2A

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UniProt ID: (Human) P05549

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Entrez Gene ID: (Human) 7020, (Rat) 306862

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription coactivator activity protein binding transcription factor binding protein homodimerization activity sequence-specific DNA binding transcription regulatory region DNA binding protein dimerization activity RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription coactivator activity RNA polymerase II transcription corepressor activity transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding general transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter kidney development optic vesicle morphogenesis optic cup structural organization transcription from RNA polymerase II promoter sensory perception of sound negative regulation of cell proliferation response to water deprivation positive regulation of gene expression retina layer formation negative regulation of transcription by competitive promoter binding Schwann cell development trigeminal nerve development oculomotor nerve formation positive regulation of bone mineralization response to lipopolysaccharide embryonic forelimb morphogenesis inner ear morphogenesis negative regulation of apoptotic process positive regulation of neuron apoptotic process regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic cranial skeleton morphogenesis palate development bone morphogenesis eyelid development in camera-type eye positive regulation of tooth mineralization cellular response to iron ion negative regulation of reactive oxygen species metabolic process neural tube closure lens morphogenesis in camera-type eye outflow tract morphogenesis keratinocyte development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter embryonic pattern specification response to organic substance embryonic body morphogenesis neural crest cell development anterior neuropore closure forebrain neuron development positive regulation of cell migration forelimb morphogenesis negative regulation of epidermal growth factor receptor signaling pathway regulation of cell proliferation negative regulation of neuron apoptotic process skin development regulation of neuron differentiation sympathetic nervous system development embryonic camera-type eye morphogenesis skeletal system morphogenesis epidermis morphogenesis lens induction in camera-type eye face morphogenesis cornea development in camera-type eye basement membrane organization metanephric nephron development
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