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Invitrogen
HEF1 Polyclonal Antibody
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Product Details
PA5-83453
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human NEDD9. Recombinant protein control fragment (Product #RP-104700).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: PPMRQAGRPD LRPEGVYDIP PTCTKPAGKD LHVKYNCDIP GAAEPVARRH QSLSPNHPPP QLGQSVGSQN DAYDVPRGVQ FLEPPA
Highest antigen sequence indentity to the following orthologs: Mouse - 72%, Rat - 70%.
Target Information
HEF-1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis and oncogenic transformation. The Cas family of docking proteins have been the subject of intense research because of their role in cell motility, growth, apoptosis and oncogenic transformation. These proteins are substrates of focal adhesion kinase (FAK) and the Src family of tyrosine kinases two active targets for drug development. HEF1 protein production increases levels of mRNA transcripts that encode proteins associated with motility, cell transformation and invasiveness, including several metalloproteinases, MLCK, p160ROCK and ErbBi. HEF1 overproduction also mediates apoptosis in epithelial-derived cell lines, including MCF7 and HeLa cells. Recent clinical studies at another institution have found that overexpression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance. This highlights the importance of studying the role of this family of proteins in cancer prognosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cas scaffolding protein family member 2; CAS-L; cas-like docking; CASS2; Crk-associated substrate related protein Cas-L; CRK-associated substrate-related protein; dJ49G10.2; dJ761I2.1; Enhancer of filamentation 1; Enhancer of filamentation 1 p55; hEF1; NEDD-9; Neural precursor cell expressed developmentally down-regulated protein 9; p105; p130Cas-related protein; pp105; p130Cas-related protein; Renal carcinoma antigen NY-REN-12; unnamed protein product
Gene Aliases: CAS-L; CAS2; CASL; CASS2; HEF1; NEDD9
UniProt ID: (Human) Q14511
Entrez Gene ID: (Human) 4739
cytoskeleton organization
cell adhesion
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
integrin-mediated signaling pathway
learning or memory
cell migration
positive regulation of cell migration
negative regulation of cell migration
regulation of actin cytoskeleton organization
positive regulation of osteoclast differentiation
actin filament bundle assembly
cell division
positive regulation of protein tyrosine kinase activity
cilium disassembly
lymphocyte migration into lymphoid organs
positive regulation of lymphocyte chemotaxis
positive regulation of substrate adhesion-dependent cell spreading
positive regulation of dendritic spine maintenance
positive regulation of cellular protein localization
positive regulation of immunological synapse formation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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