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Invitrogen
PIT1 Polyclonal Antibody
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FIGURE: 1 / 9
PIT1 Antibody (PA5-84020) in ICC/IF
Immunofluorescence analysis of PIT1/POU1F1 was performed using 70% confluent log phase A-673 and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PIT1 Polyclonal Antibody (Product # PA5-84020, 1:100 dilution) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Prod... View More
Product Details
PA5-84020
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human POU1F1. Recombinant protein control fragment (Product #RP-106298).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: FPDHTLSHGF PPIHQPLLAE DPTAADFKQE LRRKSKLVEE PIDMDSPEIR ELEKFANEFK VRRIKLGYTQ TNVGEALAAV HGS
Highest antigen sequence indentity to the following orthologs: Rat - 95%, Mouse - 94%.
Target Information
PIT1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were PIT1 and OCT1 of mammals, and Unc-86 of C. elegans. PIT1 contains 2 protein domains, termed POU-specific and POU-homeo, which are both necessary for high affinity DNA binding on genes encoding growth hormone and prolactin. PIT1 is also important for regulation of the genes encoding prolactin and thyroid-stimulating hormone beta subunit by thyrotropin-releasing hormone and cyclic AMP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GHF-1; Growth hormone factor 1; PIT-1; Pituitary-specific positive transcription factor 1; POU domain, class 1, transcription factor 1
Gene Aliases: CPHD1; GHF-1; GHF1; Pit-1; PIT1; POU1F1; POU1F1a
UniProt ID: (Human) P28069
Entrez Gene ID: (Human) 5449
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
RNA polymerase II activating transcription factor binding
RNA polymerase II transcription coactivator activity
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
negative regulation of transcription from RNA polymerase II promoter
cell fate specification
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
positive regulation of cell proliferation
negative regulation of cell proliferation
determination of adult lifespan
B cell differentiation
positive regulation of inositol trisphosphate biosynthetic process
positive regulation of multicellular organism growth
regulation of insulin-like growth factor receptor signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
nuclear transport
somatotropin secreting cell development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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