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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • UBE2M Antibodies

          Invitrogen

          UBE2M Polyclonal Antibody

          View all (15) UBE2M antibodies

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          Cite UBE2M Polyclonal Antibody

          • Antibody Testing Data (3)
          UBE2M Antibody in Immunocytochemistry (ICC/IF)
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          UBE2M Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          UBE2M Antibody (PA5-84357) in ICC/IF

          Immunofluorecent analysis of UBE2M in human cell line MCF7 using UBE2M Polyclonal Antibody (Product # PA5-84357). Staining shows localization to nucleus and nucleoli. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          UBE2M Antibody in Immunocytochemistry (ICC/IF)
          UBE2M Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          UBE2M Antibody in Western Blot (WB)
          UBE2M Polyclonal Antibody

          Product Details

          PA5-84357

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.04-0.4 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:50
          -

          Immunocytochemistry (ICC/IF)

          0.25-2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein corresponding to Human UBE2M. Recombinant protein control fragment (Product #RP-104019).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.05 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 40% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2791509

          Product Specific Information

          Immunogen sequence: KLFSLKQQKK EEESAGGTKG SSKKASAAQL RIQKDINELN LPKTCDISFS DPDDLLNFKL VICPDEGFYK SGKFVFSFKV GQ

          Highest antigen sequence indentity to the following orthologs: Rat - 100%, Mouse - 100%.

          Target Information

          The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: NEDD8 carrier protein; NEDD8 protein ligase; NEDD8-conjugating enzyme Ubc12; ubiquitin carrier protein M; ubiquitin conjugating enzyme E2M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog

          View more View less

          Gene Aliases: hUbc12; UBC-RS2; UBC12; UBE2M

          View more View less

          UniProt ID: (Human) P61081

          View more View less

          Entrez Gene ID: (Human) 9040

          View more View less

          Function(s)
          ubiquitin-protein transferase activity protein binding ATP binding ribosomal S6-glutamic acid ligase activity NEDD8 transferase activity ubiquitin protein ligase binding ubiquitin protein ligase activity ubiquitin-protein ligase
          Process(es)
          cellular protein modification process transforming growth factor beta receptor signaling pathway protein ubiquitination NIK/NF-kappaB signaling positive regulation of neuron apoptotic process protein neddylation
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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