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Invitrogen
PBRM1 Polyclonal Antibody
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FIGURE: 1 / 10
PBRM1 Antibody (PA5-84414) in ICC/IF
Immunofluorescence analysis of PBRM1 was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PBRM1 Polyclonal Antibody (Product # PA5-84414) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A... View More
Product Details
PA5-84414
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human PBRM1. Recombinant protein control fragment (Product #RP-105128).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: PVIQQPTTPM FVAPPPKTQR LLHSEAYLKY IEGLSAESNS ISKWDQTLAA RRRDVHLSKE QESRLPSHWL KSKGAHTTMA DALWRLRDLM LRDTLNIR
Highest antigen sequence indentity to the following orthologs: Mouse - 100%, Rat - 100%.
Target Information
The PBRM1 gene encodes a subunit of the PBAF (Polybromo-associated BAF) chromatin remodeling complex, playing a crucial role in the regulation of chromatin structure and transcription. PBRM1, also known as BAF180, contains bromodomains that recognize acetylated lysine residues on histone tails, facilitating chromatin remodeling necessary for effective transcriptional regulation. This gene is frequently mutated in renal cell carcinoma, particularly clear cell renal cell carcinoma (ccRCC), where such mutations are implicated in the pathogenesis of the disease. PBRM1 acts as a tumor suppressor, and its loss of function can lead to deregulation of gene expression, contributing to oncogenic processes. It is involved in pathways that govern cell cycle regulation and DNA damage response, reflecting its importance in maintaining genomic stability. Ongoing research focuses on understanding PBRM1's interactions within chromatin remodeling complexes and its potential as a therapeutic target in cancer treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BAF180; BRG1-associated factor 180; contains six bromodomains; hPB1; polybromo protein; Polybromo-1D; Protein polybromo-1; unnamed protein product
Gene Aliases: BAF180; PB1; PBRM1; RCC; SMARCH1
UniProt ID: (Human) Q86U86
Entrez Gene ID: (Human) 55193
mitotic cell cycle
chromatin organization
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
negative regulation of cell proliferation
regulation of mitotic metaphase/anaphase transition
positive regulation of T cell differentiation
positive regulation of cell differentiation
positive regulation of myoblast differentiation
regulation of G0 to G1 transition
regulation of G1/S transition of mitotic cell cycle
positive regulation of double-strand break repair
regulation of nucleotide-excision repair
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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