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Invitrogen
ATM Polyclonal Antibody
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Product Details
PA5-84748
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human ATM. Recombinant protein control fragment (Product #RP-107548).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LTIVEVLLYD PLFDWTMNPL KALYLQQRPE DETELHPTLN ADDQECKRNL SDIDQSFNKV AERVLMRLQE KLKGVE
Highest antigen sequence indentity to the following orthologs: Rat - 91%, Mouse - 91%.
Target Information
Ataxia-telangiectasia Mutated (ATM) is a protein that belongs to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and an increased risk of lymphoid cancer. The ATM gene codes for a protein belonging to the phosphoinositide 3-kinase (PI3K) superfamily. ATM phosphorylates proteins instead of lipid and has many downstream targets that act as cell-cycle regulators including: P53, Mdm2, BRCA1, and SMC1. The ATM protein is responsible for repairing double-stranded DNA breaks that occur because of ionizing radiation and other mutagens. The ATM's C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Studies have shown that ATM becomes autophosphorylated and upregulated by exposure to ionizing radiation. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. Further, DNA damage caused by ionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions that regulate cell cycle, apoptosis, and DNA damage repair. Studies have linked ATM to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A-T; A-T mutated; AT mutated; AT mutated protein; Ataxia t; Ataxia telangiectasia mutated; DKFZp781A0353; EC 2.7.1.37; MGC74674; mutated in ataxia telangiectasia; Serine-protein kinase ATM; serine/threonine kinase ATM; TEL1, telomere maintenance 1, homolog; unnamed protein product
Gene Aliases: AT1; ATA; ATC; ATD; ATDC; ATE; ATM; TEL1; TELO1
UniProt ID: (Human) Q13315
Entrez Gene ID: (Human) 472
nucleotide binding
DNA binding
protein kinase activity
protein serine/threonine kinase activity
DNA-dependent protein kinase activity
protein binding
ATP binding
kinase activity
1-phosphatidylinositol-3-kinase activity
transferase activity
histone H2AXS139 kinase activity
identical protein binding
macromolecular complex binding
protein serine kinase activity
autophagosome assembly
DNA damage checkpoint
pexophagy
telomere maintenance
double-strand break repair via homologous recombination
DNA double-strand break processing
ovarian follicle development
somitogenesis
pre-B cell allelic exclusion
immune system process
DNA repair
double-strand break repair
double-strand break repair via nonhomologous end joining
chromatin remodeling
protein phosphorylation
protein monoubiquitination
cellular response to DNA damage stimulus
response to oxidative stress
mitotic spindle assembly checkpoint
mitotic G2 DNA damage checkpoint
meiosis I
reciprocal meiotic recombination
male meiosis
female meiotic division
signal transduction
female gamete generation
brain development
heart development
determination of adult lifespan
female gonad development
intrinsic apoptotic signaling pathway in response to DNA damage
post-embryonic development
response to ionizing radiation
regulation of gene expression
regulation of autophagy
positive regulation of macromolecule biosynthetic process
positive regulation of gene expression
DNA damage response, signal transduction by p53 class mediator
positive regulation of cell migration
negative regulation of B cell proliferation
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
regulation of telomere maintenance via telomerase
positive regulation of telomere maintenance via telomerase
V(D)J recombination
cellular response to stress
cellular response to reactive oxygen species
multicellular organism growth
phosphatidylinositol-3-phosphate biosynthetic process
peptidyl-serine autophosphorylation
lipoprotein catabolic process
response to starvation
signal transduction in response to DNA damage
regulation of apoptotic process
positive regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of DNA damage response, signal transduction by p53 class mediator
positive regulation of neuron apoptotic process
meiotic telomere clustering
positive regulation of cell adhesion
positive regulation of transcription from RNA polymerase II promoter
protein autophosphorylation
thymus development
oocyte development
protein stabilization
neuron apoptotic process
regulation of cell cycle
chromosome organization involved in meiotic cell cycle
protein K63-linked ubiquitination
histone mRNA catabolic process
cellular response to retinoic acid
cellular response to gamma radiation
cellular response to X-ray
cellular response to nitrosative stress
regulation of cellular response to stress
cellular senescence
replicative senescence
establishment of RNA localization to telomere
establishment of macromolecular complex localization to telomere
DNA strand resection involved in replication fork processing
regulation of cellular response to heat
cellular response to oxygen-containing compound
regulation of signal transduction by p53 class mediator
negative regulation of signal transduction by p53 class mediator
negative regulation of TORC1 signaling
negative regulation of telomere capping
positive regulation of telomere maintenance via telomere lengthening
positive regulation of telomerase catalytic core complex assembly
positive regulation of double-strand break repair via homologous recombination
protein localization to site of double-strand break
negative regulation of double-strand break repair via homologous recombination
positive regulation of double-strand break repair
regulation of autophagosome assembly
positive regulation of autophagosome assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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