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Invitrogen
MLCK Polyclonal Antibody
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Product Details
PA5-84926
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human MYLK3. Recombinant protein control fragment (Product #RP-108263).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: EEEGGKPKHV LSTSGVQSDA REPGEESQKA DVLEGTAERL PPIRASGLGA DPAQAVVSPG QGDGVPGPAQ AFPGHLPLPT KVEAK
Highest antigen sequence indentity to the following orthologs: Rat - 45%, Mouse - 49%.
Target Information
MLCK (MLCK2), now also known as MYLK3, is of the myosin regulatory light chain kinases which facilitates muscle contraction. MLCK, a member of the Ser/Thr protein kinase family, is responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cardiac-MLCK; cardiac-MyBP-C associated Ca/CaM kinase; Cardiac-MyBP-C-associated Ca/CaM kinase; MLC kinase; Myosin light chain kinase 3; putative myosin light chain kinase 3
Gene Aliases: caMLCK; MLCK; MLCK2; MYLK3
UniProt ID: (Human) Q32MK0
Entrez Gene ID: (Human) 91807
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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