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Invitrogen
HERC5 Polyclonal Antibody
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FIGURE: 1 / 3
HERC5 Antibody (PA5-100555) in IHC (P)
Immunohistochemistry analysis of HERC5 in mouse testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with HERC5 polyclonal antibody (Product # PA5-100555) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-100555
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HERC5(Accession Q9UII4), corresponding to amino acid residues G303-E353.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total HERC5.
Target Information
HERC5 (E3 ISG15 - protein ligase HERC5) is a major E3 ligase for ISG15 conjugation. It acts a positive regulator of innate antiviral response in cells induced by interferon. HERC5 functions as a part of the ISGylation machinery that recognizes target protein in a broad and relatively non-specific manner. It catalyzes ISGylation of IRF3 - which results in sustained activation, it attenuates IRF3-PIN1 interaction - which antagonizes IFR3 ubiquitination and degradation, and boosts the antiviral repsonse. HERC5 is physically assocated with polyribosomes and broadly modifies newly synthesized proteins in a cotranslational manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cyclin-E-binding protein 1; HECT domain and RCC1-like domain-containing protein 5; hect domain and RLD 5; probable E3 ubiquitin-protein ligase HERC5; ubiquitin ligase; unnamed protein product
Gene Aliases: CEB1; CEBP1
UniProt ID: (Human) Q9UII4
Entrez Gene ID: (Human) 51191
regulation of cyclin-dependent protein serine/threonine kinase activity
ubiquitin-dependent protein catabolic process
protein ubiquitination
ISG15-protein conjugation
PKR/eIFalpha signaling
innate immune response
regulation of defense response to virus
antiviral innate immune response
positive regulation of cGAS/STING signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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