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Invitrogen
EHHADH Polyclonal Antibody
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FIGURE: 1 / 3
EHHADH Antibody (PA5-101741) in IHC (P)
Immunohistochemistry analysis of EHHADH in human colorectal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with EHHADH polyclonal antibody (Product # PA5-101741) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-101741
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human EHHADH(Accession Q08426), corresponding to amino acid residues N475-A525.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total EHHADH.
Target Information
As an L-hydroxy-specific enzyme, EHHADH (enoyl-CoA-hydratase:3-hydroxyacyl-CoA dehydrogenase), also known as Peroxisomal L-bifunctional enzyme, is a 723 amino acid protein has an essential tripeptide sequence on its carboxyl-terminus that is required for peroxisomal transport. EHHADH-null mice only exhibit a blunted peroxisome proliferative response when challenged with a peroxisome proliferator. Since there were no observed changes in lipid metabolism, this evidence suggests that enoyl-CoAs were diverted to the D-hydroxy-specific beta-oxidation system for metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3,2-trans-enoyl-CoA isomerase; enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase; enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase; L-3-hydroxyacyl-CoA dehydrogenase; L-bifunctional enzyme; L-bifunctional protein, peroxisomal; L-peroxisomal bifunctional enzyme; L-specific bifunctional protein; L-specific multifunctional beta-oxdiation protein; multifunctional enzyme 1; multifunctional enzyme type 1; multifunctional protein 1; PBE; peroxisomal bifunctional enzyme type 1; peroxisomal enoyl-CoA hydratase; peroxisomal enoyl-CoA/hydrotase-3-hydroxyacyl-CoA; peroxisomal enoyl-CoA: hydrotase-3-hydroxyacyl-CoA; unnamed protein product
Gene Aliases: 1300002P22Rik; ECHD; FRTS3; HD; L-PBE; LBFP; LBP; MEF; Mfe; MFE1; MFP; MFP-1; MFP1; Pbe; PBFE; pe-CoA; perMFE-1
UniProt ID: (Human) Q08426, (Mouse) Q9DBM2, (Rat) P07896
Entrez Gene ID: (Human) 1962, (Mouse) 74147, (Rat) 171142
catalytic activity
3-hydroxyacyl-CoA dehydrogenase activity
dodecenoyl-CoA delta-isomerase activity
enoyl-CoA hydratase activity
protein binding
oxidoreductase activity
long-chain-3-hydroxyacyl-CoA dehydrogenase activity
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
intramolecular oxidoreductase activity, transposing C=C bonds
3-hydroxyacyl-CoA dehydratase activity
enzyme binding
NAD+ binding
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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