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Invitrogen
GCLC Polyclonal Antibody
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FIGURE: 1 / 3
GCLC Antibody (PA5-103134) in ICC/IF
Immunofluorescent analysis of GCLC in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and GCLC polyclonal antibody (Product # PA5-103134) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-103134
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GCLC(Accession P48506), corresponding to amino acid residues K73-N123.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total GCLC.
Target Information
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: gamma GCS-HS; Gamma-ECS; Gamma-glutamylcysteine synthetase; gamma-glutamylcysteine synthetase heavy subunit; GCS heavy chain; Glutamate--cysteine ligase catalytic subunit; Glutamylcysteine gamma synthetase light chain; unnamed protein product
Gene Aliases: CNSHA7; D9Wsu168e; GCL; GCLC; GCS; Ggcs-hs; GLCL; GLCL-H; GLCLC
UniProt ID: (Human) P48506, (Rat) P19468, (Mouse) P97494
Entrez Gene ID: (Pig) 100522018, (Human) 2729, (Rat) 25283, (Mouse) 14629
cysteine metabolic process
glutamate metabolic process
glutathione metabolic process
glutathione biosynthetic process
response to oxidative stress
response to nutrient
response to heat
response to xenobiotic stimulus
response to hormone
response to activity
L-ascorbic acid metabolic process
negative regulation of protein ubiquitination
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
cellular response to insulin stimulus
cellular response to hepatocyte growth factor stimulus
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
cellular response to fibroblast growth factor stimulus
response to human chorionic gonadotropin
cell redox homeostasis
negative regulation of transcription, DNA-templated
response to arsenic-containing substance
response to cadmium ion
response to nitrosative stress
regulation of mitochondrial depolarization
response to interleukin-1
cellular response to mechanical stimulus
cellular response to glucose stimulus
cellular response to follicle-stimulating hormone stimulus
cellular response to thyroxine stimulus
regulation of blood vessel diameter
negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
negative regulation of hepatic stellate cell activation
negative regulation of extrinsic apoptotic signaling pathway
apoptotic mitochondrial changes
regulation of blood vessel size
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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