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Invitrogen
PRSS3 Polyclonal Antibody
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FIGURE: 1 / 5
PRSS3 Antibody (PA5-103175) in ICC/IF
Immunocytochemistry analysis of PRSS3 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PRSS3 polyclonal antibody (Product # PA5-103175) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-103175
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PRSS3(Accession P35030), corresponding to amino acid residues P9-A59.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Trypsin-3.
Target Information
PRSS3 encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. PRSS3 is localized to the locus of T cell receptor beta variable orphans on chromosome 9. Four transcript variants encoding different isoforms have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: brain trypsinogen; mesotrypsin; mesotrypsinogen; pancreatic trypsinogen III; protease, serine 3; protease, serine, 4 (trypsin 4, brain); PRSS4B; trypsin 4; trypsin 3; trypsin III; trypsin IV; trypsinogen; trypsinogen 15; trypsinogen 4; trypsinogen 5; trypsinogen IV; unnamed protein product
Gene Aliases: MTG; PRSS4; T9; Tb; TRY3; TRY4
UniProt ID: (Human) P35030
Entrez Gene ID: (Human) 5646, (Mouse) 22073
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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