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Invitrogen

SMAD1/SMAD5/SMAD9 Polyclonal Antibody

View all (8) SMAD1/SMAD5/SMAD9 antibodies

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SMAD1/SMAD5/SMAD9 Polyclonal Antibody

Product Details

PA5-104523

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human SMAD1(Accession Q15797), corresponding to amino acid residues I438-S465.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2853824

Product Specific Information

Antibody detects endogenous levels of total Smad1/5/9.

Target Information

The protein encoded by SMAD1/SMAD5/SMAD8/SMAD9 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: chromosome 5 Mad homolog; dwarfin-A; dwarfin-C; homolog of Drosophila Mothers against dpp (Mad); MAD (mothers against decapentaplegic, Drosophila) homolog 1; MAD homolog 1; MAD homolog 5; MAD homolog 9; MAD homolog1 (mothers against decapentaplegic, Drosophila); MAD, mothers against decapentaplegic homolog 1; MAD, mothers against decapentaplegic homolog 5; Mad-related protein 1; MADH5; member of receptor-regulated Smad family; mothers against decapentaplegic homolog 1; mothers against decapentaplegic homolog 5; mothers against decapentaplegic homolog 9; mothers against decapentaplegic, drosophila, homolog of, 5; Mothers against decapentaplegic, drosophila, homolog of, 9; mothers against DPP homolog 1; mothers against DPP homolog 5; mothers against DPP homolog 9; mothers-against-DPP-related 1; mSmad5; Sma and Mad homolog; SMA- and MAD-related protein 5; SMAD; SMAD 1; SMAD 5; SMAD 9; SMAD, mothers against DPP homolog 1; SMAD, mothers against DPP homolog 5; SMAD, mothers against DPP homolog 9; Smad5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; unnamed protein product

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Gene Aliases: 1110051M15Rik; BSP-1; BSP1; dwf-A; Dwf-C; DWFC; JV4-1; JV41; JV5-1; Mad1; MADH1; MADH5; MADH6; Madh8; MADH9; MADR1; Mlp1; mMad1; MusMLP; PPH2; SMAD8; SMAD8/9; SMAD8A; SMAD8B

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UniProt ID: (Human) Q15797, (Human) Q99717, (Human) O15198, (Rat) P97588, (Mouse) P70340, (Mouse) P97454, (Rat) Q9R1V3, (Mouse) Q9JIW5, (Rat) O54835

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Entrez Gene ID: (Human) 4086, (Human) 4090, (Human) 4093, (Rat) 25671, (Mouse) 17125, (Mouse) 17129, (Rat) 59328, (Mouse) 55994, (Rat) 85435

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding DEAD/H-box RNA helicase binding protein kinase binding ubiquitin protein ligase binding identical protein binding sequence-specific DNA binding co-SMAD binding I-SMAD binding primary miRNA binding RNA polymerase II regulatory region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding sequence-specific double-stranded DNA binding
Process(es)
ossification osteoblast differentiation ureteric bud development cardiac conduction system development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cellular iron ion homeostasis inflammatory response signal transduction transforming growth factor beta receptor signaling pathway anatomical structure morphogenesis embryonic pattern specification cell differentiation BMP signaling pathway primary miRNA processing developmental process positive regulation of transcription from RNA polymerase II promoter stem cell differentiation SMAD protein signal transduction cellular response to growth factor stimulus transforming growth factor beta receptor superfamily signaling pathway positive regulation of dendrite development positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of sprouting angiogenesis anti-Mullerian hormone signaling pathway cellular response to BMP stimulus MAPK cascade mesodermal cell fate commitment kidney development osteoblast fate commitment protein phosphorylation gamete generation negative regulation of cell proliferation response to xenobiotic stimulus positive regulation of gene expression negative regulation of muscle cell apoptotic process cardiac muscle hypertrophy in response to stress midbrain development hindbrain development homeostatic process positive regulation of cell differentiation positive regulation of osteoblast differentiation positive regulation of dendrite morphogenesis cartilage development cardiac muscle cell proliferation bone development positive regulation of cartilage development skeletal system development Mullerian duct regression positive regulation of transcription, DNA-templated negative regulation of transcription from RNA polymerase II promoter germ cell development negative regulation of gene expression erythrocyte differentiation negative regulation of apoptotic process cardiac muscle contraction negative regulation of Fas signaling pathway regulation of ossification response to hypoxia
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