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Invitrogen
Phospho-ULK1 (Ser317) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-ULK1 (Ser317) Antibody (PA5-104556) in ICC/IF
Immunocytochemistry analysis of Phospho-ULK1 (Ser317) in Hela cells (heat shock treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-ULK1 (Ser317) polyclonal antibody (Product # PA5-104556) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit I... View More
Product Details
PA5-104556
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ULK1(Accession O75385), corresponding to amino acid residues around phosphorylated Ser317.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of ULK1 only when phosphorylated at Ser317.
Target Information
ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APG1; ATG 1; ATG1; ATG1 autophagy related 1 homolog; Autophagy-related protein 1 homolog; C. elegans; EC:2.7.11.1; FLJ38455; Serine/threonine-protein kinase ULK1; Serine/threonine-protein kinase Unc51.1; ULK 1; UNC 51; Unc-51-like kinase 1
Gene Aliases: ATG1; ATG1A; AU041434; hATG1; KIAA0722; mKIAA0722; ULK1; UNC51; Unc51.1
UniProt ID: (Human) O75385, (Mouse) O70405
Entrez Gene ID: (Human) 8408, (Rat) 360827, (Mouse) 22241
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
small GTPase binding
identical protein binding
macromolecular complex binding
GTPase binding
protein serine kinase activity
molecular_function
Rab GTPase binding
protein kinase binding
protein complex binding
transferase activity, transferring phosphorus-containing groups
Hsp90 protein binding
non-receptor serine/threonine protein kinase
autophagosome assembly
mitophagy
protein phosphorylation
ubiquitin-dependent protein catabolic process
autophagy
signal transduction
axonogenesis
protein localization
negative regulation of cell proliferation
regulation of autophagy
positive regulation of autophagy
regulation of tumor necrosis factor-mediated signaling pathway
macroautophagy
regulation of macroautophagy
peptidyl-serine phosphorylation
neuron projection regeneration
neuron projection development
negative regulation of protein complex assembly
cellular response to nutrient levels
cellular response to stress
piecemeal microautophagy of nucleus
response to starvation
protein autophosphorylation
negative regulation of collateral sprouting
axon extension
reticulophagy
protein-containing complex assembly
positive regulation of autophagosome assembly
Ras protein signal transduction
positive regulation of macroautophagy
cerebellar granule cell differentiation
radial glia guided migration of cerebellar granule cell
receptor internalization
regulation of neurotrophin TRK receptor signaling pathway
regulation of gene expression
regulation of cell death
phosphorylation
mitophagy in response to mitochondrial depolarization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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