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Invitrogen
Phospho-Connexin 43 (Ser262) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-Connexin 43 (Ser262) Antibody (PA5-104562) in WB
Western blot analysis of Phospho-Connexin 43 (Ser262) in extracts from SNB19 cells treated with TPA. Samples were incubated with Phospho-Connexin 43 (Ser262) polyclonal antibody (Product # PA5-104562). Lane 1 was treated with phospho-blocking peptide, Lane 2 was treated with non-phospho-blocking peptide.
Product Details
PA5-104562
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GJA1(Accession P17302), corresponding to amino acid residues around phosphorylated Ser262.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of Connexin 43 / GJA1 only when phosphorylated at Ser262.
Target Information
Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Connexin-43; Cx-43; Cx43; CXA1; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction protein, alpha 1, 43kDa; GJA1; ODD; ODOD; SDTY3; Syndactyly type III; unnamed protein product
Gene Aliases: AVSD3; CMDR; CX43; EKVP; EKVP3; GJA1; GJAL; HLHS1; HSS; ODDD; PPKCA
UniProt ID: (Human) P17302
Entrez Gene ID: (Human) 2697
gap junction channel activity
protein binding
beta-catenin binding
ion transmembrane transporter activity
efflux transmembrane transporter activity
tubulin binding
transmembrane transporter activity
glutathione transmembrane transporter activity
alpha-tubulin binding
gap junction hemi-channel activity
gap junction channel activity involved in cardiac conduction electrical coupling
gap junction channel activity involved in cell communication by electrical coupling
establishment of mitotic spindle orientation
cardiac conduction system development
cell communication
signal transduction
cell-cell signaling
spermatogenesis
heart development
protein localization
positive regulation of gene expression
cell communication by electrical coupling
glutamate secretion
gap junction assembly
negative regulation of cell growth
negative regulation of gonadotropin secretion
ion transmembrane transport
glutathione transmembrane transport
maintenance of blood-brain barrier
xenobiotic transport
positive regulation of I-kappaB kinase/NF-kappaB signaling
cell-cell junction organization
bone remodeling
transmembrane transport
bone development
atrial cardiac muscle cell action potential
cell communication by electrical coupling involved in cardiac conduction
microtubule-based transport
positive regulation of cold-induced thermogenesis
export across plasma membrane
negative regulation of trophoblast cell migration
regulation of blood circulation
cellular response to beta-amyloid
positive regulation of vascular smooth muscle cell proliferation
positive regulation of morphogenesis of an epithelium
positive regulation of mesodermal cell differentiation
positive regulation of stem cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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