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Invitrogen

Phospho-IKK beta (Tyr199) Polyclonal Antibody

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Phospho-IKK beta (Tyr199) Polyclonal Antibody

Product Details

PA5-104694

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human IKBKB(Accession O14920), corresponding to amino acid residues around phosphorylated Tyr199.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816167

Product Specific Information

Antibody detects endogenous levels of IKK beta only when phosphorylated at Tyrosine 199.

Target Information

Phosphorylation of serine residues on the I-kappa-B proteins by IKK kinases, including IKBKB, marks them for destruction via the ubiquitination pathway, allowing activation of the NF-kappa-B complex. Inhibitor of nuclear factor kappa-B kinase subunit beta (IKK beta) is a serine kinase that plays an essential role in the NF-kaapa-B signaling pathway which is activated by mulitple stimuli such as inflammatory cytokines, bacterial, or viral products, DNA damages, or other cellular stresses. IKK beta acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Mutations affecting the IKK beta gene can result in Immunodeficiency 15 (IMD15).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ40509; I-kappa-B kinase 2; I-kappa-B-kinase beta; IKK-B; IKK-beta; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of kappaB kinase beta; inhibitor of nuclear factor kappa B kinase beta subunit; MGC131801; NFKBIKB; nuclear factor kappa B kinase subunit beta; nuclear factor NF-kappa-B inhibitor kinase beta; serine/threonine protein kinase IKBKB; unnamed protein product

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Gene Aliases: AIM-1; IKK-2; IKK-B; IKK-beta; IKK2; IKK[b]; IKKB; IKKbeta; IMD15; IMD15A; IMD15B; NFKBIKB

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UniProt ID: (Human) O14920, (Mouse) O88351, (Rat) Q9QY78

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Entrez Gene ID: (Human) 3551, (Mouse) 16150, (Rat) 84351

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein binding ATP binding IkappaB kinase activity protein kinase binding identical protein binding protein homodimerization activity protein heterodimerization activity scaffold protein binding protein serine kinase activity transferrin receptor binding protein phosphatase binding macromolecular complex binding
Process(es)
protein polyubiquitination B cell homeostasis regulation of transcription from RNA polymerase II promoter protein phosphorylation inflammatory response integrin-mediated signaling pathway I-kappaB kinase/NF-kappaB signaling I-kappaB phosphorylation positive regulation of cell proliferation positive regulation of sodium ion transport positive regulation of neuron projection development peptidyl-serine phosphorylation signal transduction involved in regulation of gene expression neuron projection development tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter protein maturation response to cholecystokinin interleukin-1-mediated signaling pathway cellular response to tumor necrosis factor protein localization to plasma membrane negative regulation of cytokine production involved in inflammatory response regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly pattern recognition receptor signaling pathway stimulatory C-type lectin receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent MyD88-dependent toll-like receptor signaling pathway response to virus regulation of tumor necrosis factor-mediated signaling pathway regulation of toll-like receptor signaling pathway toll-like receptor 3 signaling pathway TRIF-dependent toll-like receptor signaling pathway Fc-epsilon receptor signaling pathway innate immune response T cell receptor signaling pathway stress-activated MAPK cascade skeletal muscle contraction response to xenobiotic stimulus response to toxic substance response to lipopolysaccharide response to fatty acid
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