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Invitrogen
Phospho-NCF2 (Thr223) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-NCF2 (Thr223) Antibody (PA5-105094) in ICC/IF
Immunocytochemistry analysis of Phospho-NCF2 (Thr223) in HepG2 cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-NCF2 (Thr223) polyclonal antibody (Product # PA5-105094) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105094
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NCF2(Accession P19878), corresponding to amino acid residues around phosphorylated Thr233.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of NCF2 only when phosphorylated at Thr233.
Target Information
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Defects in NCF2 are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 2 (CGD2). Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 67 kDa neutrophil oxidase factor; chronic granulomatous disease, autosomal 2; FLJ93058; NADPH oxidase activator 2; NADPH oxidase subunit (67kDa); NADPH oxidase subunit 67 kD; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2); neutrophil NADPH oxidase factor 2; unnamed protein product
Gene Aliases: NCF-2; NOXA2; P67-PHOX; P67PHOX
UniProt ID: (Human) P19878, (Mouse) O70145
Entrez Gene ID: (Human) 4688, (Mouse) 17970, (Rat) 364018
superoxide metabolic process
phagocytosis
cellular defense response
superoxide anion generation
innate immune response
respiratory burst
NADP catabolic process
response to glucose
response to lipopolysaccharide
cellular response to hormone stimulus
positive regulation of neuron apoptotic process
positive regulation of blood pressure
regulation of reactive oxygen species biosynthetic process
response to xenobiotic stimulus
response to activity
response to progesterone
response to lipid
response to laminar fluid shear stress
response to hyperoxia
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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