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Invitrogen

Phospho-c-Kit (Tyr721) Polyclonal Antibody

View all (199) c-Kit (CD117) antibodies

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Phospho-c-Kit (Tyr721) Polyclonal Antibody

Product Details

PA5-105144

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human KIT(Accession P10721), corresponding to amino acid residues around phosphorylated Tyr721.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816617

Product Specific Information

Antibody detects endogenous levels of c-Kit only when phosphorylated at Tyr721.

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: belly-spot; c-kit proto-oncogene protein; c-Kit protooncogene; c-kit receptor tyrosine kinase; ckit; dominant spotting; Dominant white spotting; Kit oncogene; p145 c-kit; piebald trait protein; protein kinase; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; similar to v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene; spotted sterile male; Steel Factor Receptor; tyrosine kinase; tyrosine-protein kinase Kit; unnamed protein product; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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Gene Aliases: Bs; C-Kit; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; MASTC; PBT; SCFR; SCO1; SCO5; SOW3; Ssm; Tr-kit; W

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UniProt ID: (Human) P10721, (Mouse) P05532

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Entrez Gene ID: (Human) 3815, (Rat) 64030, (Mouse) 16590

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Function(s)
protease binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity stem cell factor receptor activity protein binding ATP binding growth factor binding cytokine binding SH2 domain binding protein homodimerization activity nucleotide binding enzyme binding metal ion binding
Process(es)
ovarian follicle development hematopoietic progenitor cell differentiation myeloid progenitor cell differentiation lymphoid progenitor cell differentiation immature B cell differentiation mast cell chemotaxis myeloid leukocyte differentiation positive regulation of cytokine production involved in immune response positive regulation of dendritic cell cytokine production protein phosphorylation glycosphingolipid metabolic process chemotaxis response to stress inflammatory response transmembrane receptor protein tyrosine kinase signaling pathway spermatogenesis spermatid development positive regulation of cell proliferation germ cell migration regulation of cell shape gonad development visual learning male gonad development response to radiation positive regulation of gene expression cell migration peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway lamellipodium assembly actin cytoskeleton organization hemopoiesis B cell differentiation T cell differentiation erythrocyte differentiation melanocyte differentiation positive regulation of cell migration positive regulation of pseudopodium assembly positive regulation of mast cell cytokine production somatic stem cell population maintenance embryonic hemopoiesis ectopic germ cell programmed cell death organ growth intracellular signal transduction hematopoietic stem cell migration megakaryocyte development Fc receptor signaling pathway Kit signaling pathway erythropoietin-mediated signaling pathway positive regulation of tyrosine phosphorylation of STAT protein negative regulation of programmed cell death mast cell degranulation positive regulation of MAP kinase activity positive regulation of MAPK cascade pigmentation tongue development leukocyte activation positive regulation of Notch signaling pathway positive regulation of JAK-STAT cascade response to cadmium ion protein autophosphorylation developmental pigmentation positive regulation of long-term neuronal synaptic plasticity digestive tract development stem cell differentiation epithelial cell proliferation regulation of developmental process detection of mechanical stimulus involved in sensory perception of sound cell chemotaxis mast cell differentiation response to growth factor melanocyte migration melanocyte adhesion positive regulation of pyloric antrum smooth muscle contraction positive regulation of colon smooth muscle contraction positive regulation of small intestine smooth muscle contraction positive regulation of vascular smooth muscle cell differentiation signal transduction stem cell population maintenance regulation of cell proliferation positive regulation of protein kinase B signaling mast cell proliferation germ cell development cell differentiation
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