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Invitrogen
Phospho-NFAT5 (Ser134) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-NFAT5 (Ser134) Antibody (PA5-105437) in ICC/IF
Immunocytochemistry analysis of Phospho-NFAT5 (Ser134) in H2O2 treated HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-NFAT5 (Ser134) polyclonal antibody (Product # PA5-105437) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L)... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105437
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NFAT5(Accession O94916), corresponding to amino acid residues around phosphorylated Ser134.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of NFAT5 only when phosphorylated at Ser134.
Target Information
The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glutamine rich protein H65; NFAT-like protein 1; NFAT5b; nuclear factor of activated T-cells 5, tonicity-responsive; osmotic response element-binding protein; rel domain-containing transcription factor NFAT5; rel similarity domain containing protein; T-cell transcription factor NFAT5; TonE-binding protein; TonEBP; transcription factor; tonicity-responsive enhancer binding protein; tonicity-responsive enhancer-binding protein; transcription factor; NFAT5; unnamed protein product
Gene Aliases: B130038B15Rik; CAG-8; CAG80; mKIAA0827; NF-AT5; NFATL1; NFATZ; OREBP; TONEBP
UniProt ID: (Human) O94916, (Mouse) Q9WV30
Entrez Gene ID: (Human) 10725, (Mouse) 54446
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific double-stranded DNA binding
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
response to stress
cellular response to DNA damage stimulus
signal transduction
calcineurin-NFAT signaling cascade
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription from RNA polymerase II promoter
R-loop processing
positive regulation of mitochondrial translation
cellular response to cytokine stimulus
cellular hyperosmotic response
regulation of intracellular signal transduction
positive regulation of leukocyte adhesion to vascular endothelial cell
renal water homeostasis
response to water deprivation
positive regulation of gene expression
negative regulation of urine volume
regulation of calcineurin-NFAT signaling cascade
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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