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Invitrogen
Phospho-C/EBP epsilon (Ser181) Polyclonal Antibody
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FIGURE: 1 / 4
Phospho-C/EBP epsilon (Ser181) Antibody (PA5-105657) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-C/EBP epsilon (Ser181) in human lung cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-C/EBP epsilon (Ser181) polyclonal antibody (Product # PA5-105657) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-105657
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CEBPE(Accession Q15744), corresponding to amino acid residues around phosphorylated Ser181.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of C/EBP epsilon only when phosphorylated at ser181.
Target Information
C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP alpha appears to function exclusively in terminally-differentiated, growth-arrested cells. Additional family members include C/EBP beta, C/EBP gamma, C/EBP delta and C/EBP epsilon, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha. Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C EBP; C/EBP epsilon; C/EBP-related protein 1; C/EBPepsilon; CCAAT/enhancer binding protein (C/EBP), epsilon; CCAAT/enhancer binding protein , epsilon; CCAAT/enhancer binding protein epsilon; CCAAT/enhancer-binding protein epsilon
Gene Aliases: c/EBP epsilon; C/EBP-epsilon; C/EBPe; C/EPBe; CEBPE; CRP1; Gm294; IMD108; SGD1
UniProt ID: (Human) Q15744, (Rat) P56261, (Mouse) Q6PZD9
Entrez Gene ID: (Human) 1053, (Rat) 25410, (Mouse) 110794
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
macromolecular complex binding
sequence-specific double-stranded DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein homodimerization activity
protein heterodimerization activity
sequence-specific DNA binding
basic leucine zipper transcription factor
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
phagocytosis
defense response
positive regulation of gene expression
myeloid cell differentiation
macrophage differentiation
granulocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
cellular response to lipopolysaccharide
integrated stress response signaling
cytokine biosynthetic process
defense response to bacterium
transcription from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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