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Invitrogen
SETD8 Polyclonal Antibody
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FIGURE: 1 / 4
SETD8 Antibody (PA5-105716) in ICC/IF
Immunofluorescent analysis of SETD8 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and SETD8 polyclonal antibody (Product # PA5-105716) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105716
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human KMT5A(Accession Q9NQR1), corresponding to amino acid residues E74-C124.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total KMT5A.
Target Information
SETD8 is a histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20' monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. The protein mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It is required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. It is also involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H4-K20-HMTase; H4-K20-HMTase KMT5A; H4-K20-HMTase SETD8; H4-K20-specific histone methyltransferase; histone methyltransferase Pr-set7/Set8; histone-lysine N-methyltransferase KMT5A; histone-lysine N-methyltransferase SETD8; lysine (K)-specific methyltransferase 5A; lysine N-methyltransferase 5A; lysine-specific methylase 5A; N-lysine methyltransferase SETD8; nucleosomal histone H4-K20 methyltransferase; H4-K20-HMTase; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; SET domain containing (lysine methyltransferase) 8; SET domain-containing 8; SET domain-containing protein 8; unnamed protein product
Gene Aliases: 2410195B05Rik; PR-SET7; PR/SET07; RGD1305893; SET07; SET8; SETD8
UniProt ID: (Human) Q9NQR1, (Mouse) Q2YDW7
Entrez Gene ID: (Human) 387893, (Rat) 689820, (Mouse) 67956
transcription corepressor activity
protein binding
lysine N-methyltransferase activity
protein-lysine N-methyltransferase activity
histone methyltransferase activity
histone methyltransferase activity (H4-K20 specific)
histone H4 methyltransferase activity
histone H4K20 monomethyltransferase activity
negative regulation of transcription from RNA polymerase II promoter
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
biological_process
gene expression
peptidyl-lysine monomethylation
regulation of DNA damage response, signal transduction by p53 class mediator
negative regulation of transcription, DNA-templated
negative regulation of double-strand break repair via homologous recombination
double-strand break repair via homologous recombination
replication fork processing
protein localization to chromatin
mitotic chromosome condensation
regulation of signal transduction by p53 class mediator
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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