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Invitrogen
Phospho-CD150 (Tyr327) Polyclonal Antibody
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FIGURE: 1 / 4
Phospho-CD150 (Tyr327) Antibody (PA5-105781) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-CD150 (Tyr327) in human breast tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-CD150 (Tyr327) polyclonal antibody (Product # PA5-105781) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105781
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SLAMF1(Accession Q13291), corresponding to amino acid residues around phosphorylated Tyr327.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of SLAM / CD150 only when phosphorylated at Tyr327.
Target Information
CD150, also known as SLAM (Signaling Lymphocyte Activation Molecule), is a member of the immunoglobulin gene superfamily and plays a crucial role in T cell stimulation and immune cell interactions. It is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells. CD150 expression is rapidly upregulated upon activation, particularly on naive T cells, Th1 cells, and activated B cells, which express the membrane-bound, soluble, and cytoplasmic isoforms of SLAM. In T cells, signaling through SLAM induces proliferation and enhances the interferon-gamma response. SLAM is also involved in adhesion between T cells and antigen-presenting cells, facilitating immune cell interactions. Homophilic SLAM-SLAM binding during B to B and B to T cell interactions is suggested to enhance the expansion and differentiation of activated B cells. CD150 serves as a receptor for the measles virus and acts as a co-activator on T and B cells. It interacts with SH2D1A and PTPN11 via its cytoplasmic domain, playing a role in signal transduction. Mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP), highlighting its importance in immune regulation and disease pathology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD150 antigen; CDw150; cSLAM; IPO-3; signaling lymphocyte activation molecule; SLAM; SLAM family member 1; unnamed protein product
Gene Aliases: 4933415F16; CD150; CDw150; ESTM51; IPO-3; IPO3; RGD1560634; SLAM
UniProt ID: (Human) Q13291, (Mouse) Q9QUM4
Entrez Gene ID: (Human) 6504, (Mouse) 27218, (Rat) 498286
natural killer cell differentiation
natural killer cell proliferation
negative regulation of cytokine production
myeloid dendritic cell activation involved in immune response
negative regulation of T cell cytokine production
immune response
positive regulation of cell proliferation
negative regulation of interferon-gamma production
negative regulation of interleukin-12 production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
positive regulation of interferon-gamma production
positive regulation of activated T cell proliferation
leukocyte activation
positive regulation of JNK cascade
lymphocyte activation
viral entry into host cell
positive regulation of ERK1 and ERK2 cascade
negative regulation of CD40 signaling pathway
positive regulation of T-helper 1 cell cytokine production
leukocyte chemotaxis involved in inflammatory response
positive regulation of macrophage chemotaxis
regulation of vesicle fusion
regulation of catalytic activity
positive regulation of dendritic cell chemotaxis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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