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Invitrogen
Phospho-MCL-1 (Thr163) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-MCL-1 (Thr163) Antibody (PA5-106009) in WB
Western blot analysis of Phospho-MCL1 (Thr163) in PMA treated A549 cell lysate (absence (-) or presence (+) of non-phospho and phospho peptide). Samples were incubated with Phospho-MCL1 (Thr163) polyclonal antibody (Product # PA5-106009).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-106009
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MCL1(Accession Q07820), corresponding to amino acid residues around phosphorylated Thr163.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of MCL1 only when phosphorylated at Thr163.
Target Information
MCL1 (Myeloid cell leukemia-1) belongs to the Bcl-2 family and is involved in programing, differentiation and concomitant maintenance of cell viability, but not of proliferation. Isoform 1 of MCL1 inhibits apoptosis while isoform 2 promotes it. The carboxy terminal of MCL1 and bcl-2 share significant sequence homology. Expression of MCL1 is increased upon exposure of ML-1 cells to various types of DNA damaging agents (e.g. ionizing radiation, ultraviolet radiation, and alkylating drugs) along with increases in GADD45 and Bax and a decrease in bcl-2. Enhanced expression of MCL1, prominently associated with mitochondria, complements the continued expression of bcl-2 in ML-1 cells undergoing differentiation. Like bcl-2, MCL1 has the capacity to promote cell viability under conditions that otherwise cause apoptosis. While the mechanism by which MCL1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. MCL1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. MCL1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. MCL1 exists as at least two distinct isoforms designated MCL1L and MCL1S. In marked contrast to the larger isoform of MCL1, overexpression of MCL1S promotes cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: anti-apoptotic; apoptosis regulator protein of the Bcl-2 family; bcl-2-like protein 3; bcl-2-related protein EAT/mcl1; BCL2 family apoptosis regulator; EAT/MCL-1; MCL-1ES; MCL-1S; Mcl-1S; Bcl-2 family protein; proapoptotic protein; MCL1, BCL2 family apoptosis regulator; MGC104264; MGC1839; myeloid cell leukemia 1; myeloid cell leukemia ES; myeloid cell leukemia sequence 1; myeloid cell leukemia sequence 1 (BCL2-related); unnamed protein product
Gene Aliases: bcl2-L-3; BCL2L3; EAT; Gm52627; Mcl-1; MCL1-ES; mcl1/EAT; MCL1L; MCL1S; TM
UniProt ID: (Human) Q07820, (Rat) Q9Z1P3, (Mouse) P97287
Entrez Gene ID: (Human) 4170, (Rat) 60430, (Mouse) 17210
cell fate determination
release of cytochrome c from mitochondria
cellular response to DNA damage stimulus
mitochondrial fusion
intrinsic apoptotic signaling pathway in response to DNA damage
negative regulation of autophagy
programmed cell death
cellular homeostasis
response to cytokine
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of neuron apoptotic process
transmembrane transport
protein transmembrane transport
extrinsic apoptotic signaling pathway in absence of ligand
regulation of intracellular signal transduction
positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
negative regulation of anoikis
regulation of apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
apoptotic mitochondrial changes
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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