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Invitrogen
Phospho-MYLK (Tyr471) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-MYLK (Tyr471) Antibody (PA5-106099) in WB
Western blot analysis of Phospho-MYLK (Tyr471) in Na3VO4 treated HepG2 cell lysate (absence (-) or presence (+) of non-phospho and phospho peptide). Samples were incubated with Phospho-MYLK (Tyr471) polyclonal antibody (Product # PA5-106099).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-106099
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MYLK(Accession Q15746), corresponding to amino acid residues around phosphorylated Tyr471.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of MLCK only when phosphorylated at Tyr471.
Target Information
MYLK (MLCK) is a serine/threonine kinase that phosphorylates myosin regulatory light chains to assist in the production of contractile activity (myosin-actin interaction). MLCK is responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: kinase-related protein; MLCK; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase; telokin; unnamed protein product
Gene Aliases: AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210; MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK
UniProt ID: (Human) Q15746
Entrez Gene ID: (Human) 4638
protein phosphorylation
smooth muscle contraction
muscle organ development
tonic smooth muscle contraction
positive regulation of cell migration
bleb assembly
positive regulation of calcium ion transport
aorta smooth muscle tissue morphogenesis
cellular hypotonic response
positive regulation of wound healing
regulation of synaptic vesicle endocytosis
positive regulation of vascular smooth muscle contraction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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