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Invitrogen
DGKB Polyclonal Antibody
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FIGURE: 1 / 1
DGKB Antibody (PA5-109630) in WB
Western blot analysis of DGKB in extracts of various cell lines. Samples were incubated with DGKB Polyclonal antibody (Product # PA5-109630) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-109630
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 544-773 of human DGKB (NP_663733.1).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2.2 mg/mL
Amount
220 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LMKILKDIEN STEIMLDRWK FEVIPNDKDE KGDPVPYSII NNYFSIGVDA SIAHRFHIMR EKHPEKFNSR MKNKFWYFEF GTSETFSATC KKLHESVEIE CDGVQIDLIN ISLEGIAILN IPSMHGGSNL WGESKKRRSH RRIEKKGSDK RTTVTDAKEL KFASQDLSDQ LLEVVGLEGA MEMGQIYTGL KSAGRRLAQC SCVVIRTSKS LPMQIDGEPW MQTPCTVSTE
Target Information
Diacylglycerol (DAG) is an allosteric activator of protein kinase C. DAG also participates in regulating RAS and RHO family proteins by activating the guanine nucleotide exchange factors VAV and RASGRP1. DAG is also involved in the synthesis of phospholipids and triacylglycerols. Tight regulation of DAG levels is achieved via DAG kinases (DGKs), which remove DAG by phosphorylate it to phosphatidic acid. Several mammalian isozymes of DAGK have been identified.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 90 kDa diacylglycerol kinase; DAG kinase beta; Diacylglycerol kinase 90kDa; diacylglycerol kinase beta, 90kDa; diglyceride kinase beta; unnamed protein product
Gene Aliases: 6430574F24; 90kda; C630029D13Rik; Dagk; DAGK2; DGK; DGK-beta; mKIAA0718
UniProt ID: (Rat) P49621, (Mouse) Q6NS52
Entrez Gene ID: (Rat) 54248, (Mouse) 217480
phosphatidic acid biosynthetic process
signal transduction
phospholipase C-activating G-protein coupled receptor signaling pathway
response to bacterium
intracellular signal transduction
diacylglycerol metabolic process
phosphatidic acid metabolic process
glycerolipid metabolic process
lipid phosphorylation
modulation of synaptic transmission
regulation of postsynapse organization
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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