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Invitrogen
GTF2F1 Polyclonal Antibody
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FIGURE: 1 / 1
GTF2F1 Antibody (PA5-109796) in WB
Western blot analysis of GTF2F1 in extracts of various cell lines. Samples were incubated with GTF2F1 Polyclonal antibody (Product # PA5-109796) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-109796
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 390-517 of GTF2F1 (NP_0020872)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.65 mg/mL
Amount
65 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: PSAEGGSTSS TLRAAASKLE QGKRVSEMPA AKRLRLDTGP QSLSGKSTPQ PPSGKTTPNS GDVQVTEDAV RRYLTRKPMT TKDLLKKFQT KKTGLSSEQT VNVLAQILKR LNPERKMIND KMHFSLKE
Target Information
TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: general transcription factor IIF 74 kDa subunit; general transcription factor IIF polypeptide 1 (74kD subunit); general transcription factor IIF, polypeptide 1, 74kDa; GTF2F1; MGC94148; OTTHUMP00000237859; TFIIF-alpha; transcription initiation factor IIF subunit alpha; transcription initiation factor RAP74; unnamed protein product
Gene Aliases: 2810405L04Rik; BTF4; RAP74; TF2F1; TFIIF; TFIIF-alpha
UniProt ID: (Human) P35269, (Mouse) Q3THK3, (Rat) Q6AY96
Entrez Gene ID: (Human) 2962, (Mouse) 98053, (Rat) 316123
RNA polymerase II general transcription initiation factor binding
TFIIF-class transcription factor binding
DNA binding
RNA binding
protein binding
obsolete general RNA polymerase II transcription factor activity
phosphatase activator activity
protein phosphatase binding
protein domain specific binding
promoter-specific chromatin binding
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
response to virus
negative regulation of protein binding
positive regulation of transcription elongation from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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