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TAF9 Polyclonal Antibody
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FIGURE: 1 / 1
TAF9 Antibody (PA5-110457) in WB
Western blot analysis of TAF9 in extracts of various cell lines. Samples were incubated with TAF9 Polyclonal antibody (Product # PA5-110457) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-110457
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human TAF9 (NP_0031781)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.45 mg/mL
Amount
45 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MESGKTASPK SMPKDAQMMA QILKDMGITE YEPRVINQML EFAFRYVTTI LDDAKIYSSH AKKATVDADD VRLAIQCRAD QSFTSPPPRD FLLDIARQRN QTPLPLIKPY SGPRLPPDRY CLTAPNYRLK SLQKKASTSA GRITVPRLSV GSVTSRPSTP TLGTPTPQTM SV
Target Information
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16. A similar but distinct gene (TAF9L) has been found on the X chromosome and a pseudogene has been identified on chromosome 19. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AD-004; CGI-137; CIP; hCINAP; liver regeneration-related protein LRRG156; MGC:1603; MGC:3647; RNA polymerase II TBP-associated factor subunit G; TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor; TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa; TAF9 RNA polymerase II, TATA box binding protein-associated factor; TATA box binding protein-associated factor; transcription initiation factor TFIID 31 kD subunit; transcription initiation factor TFIID 31 kDa subunit; transcription initiation factor TFIID 32 kDa subunit
Gene Aliases: CINAP; MGC:5067; STAF31/32; TAF2G; TAFII-31; TAFII-32; TAFII31; TAFII32; TAFIID32
UniProt ID: (Human) Q16594, (Rat) Q5BKE0
Entrez Gene ID: (Human) 6880, (Rat) 373541
transcription regulatory region sequence-specific DNA binding
p53 binding
DNA binding
transcription coactivator activity
histone acetyltransferase activity
protein binding
obsolete general RNA polymerase II transcription factor activity
protein heterodimerization activity
ATPase binding
C2H2 zinc finger domain binding
DNA-binding transcription factor binding
box C/D snoRNP assembly
regulation of DNA repair
chromatin remodeling
DNA-templated transcription, initiation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
mRNA transcription from RNA polymerase II promoter
negative regulation of apoptotic process
regulation of RNA splicing
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
protein stabilization
RNA polymerase II transcriptional preinitiation complex assembly
positive regulation of transcription initiation from RNA polymerase II promoter
positive regulation of response to cytokine stimulus
response to interleukin-1
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
response to L-glutamate
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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