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Invitrogen
Phospho-Cytokeratin 8 (Ser432) Polyclonal Antibody
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FIGURE: 1 / 8
Phospho-Cytokeratin 8 (Ser432) Antibody (PA5-114566) in ICC/IF
Immunocytochemistry analysis of Phospho-Cytokeratin 8 (Ser432) in HepG2 cells (30 min of 4uM Forskolin treatment). Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114566). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red)... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-114566
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human KRT8(Accession P05787), corresponding to amino acid residues around phosphorylated Ser432.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of Cytokeratin 8 only when phosphorylated at Serine 432.
Target Information
Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CK-8; cytokeratin 8; cytokeratin endo A; cytokeratin-8; EndoA cytokeratin (5' end put.); putative; EndoA' cytokeratin (5' end put.); putative; intermediate filament; K8; keratin 8, type II; keratin complex 2, basic, gene 8; type-II keratin Kb8; unnamed protein product
Gene Aliases: CARD2; CK-8; CK8; CYK8; CYKER; EndoA; K2C8; K8; KO; Krt-2.8; Krt2-8; TROMA-1
UniProt ID: (Human) P05787, (Mouse) P11679, (Rat) Q10758
Entrez Gene ID: (Human) 3856, (Mouse) 16691, (Rat) 25626
intermediate filament organization
sarcomere organization
epithelium development
negative regulation of hepatocyte apoptotic process
tumor necrosis factor-mediated signaling pathway
response to hydrostatic pressure
response to other organism
cell differentiation involved in embryonic placenta development
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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