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Invitrogen
AKR1C1 Polyclonal Antibody
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FIGURE: 1 / 4
AKR1C1 Antibody (PA5-114836) in ICC/IF
Immunocytochemistry analysis of AKR1C1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with AKR1C1 polyclonal antibody (Product # PA5-114836) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-114836
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human AKR1C1(Accession Q04828), corresponding to amino acid residues V128-N178.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total AKR1C1.
Target Information
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 20 alpha-hydroxysteroid dehydrogenase; 20alpha-hydroxysteroid dehydrogenase; 3beta-hydroxysteroid 3-dehydrogenase; aldo-keto reductase C; chlordecone reductase homolog; chlordecone reductase homolog HAKRC; dihydrodiol dehydrogenase 1; dihydrodiol dehydrogenase 1/2; dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase; hepatic dihydrodiol dehydrogenase; high-affinity hepatic bile acid-binding protein; indanol dehydrogenase; oxidoreductase; RP11-69D4.1; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type II 3-alpha-hydroxysteroid dehydrogenase; unnamed protein product
Gene Aliases: 2-ALPHA-HSD; 20-ALPHA-HSD; DD1; DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB
UniProt ID: (Human) Q04828
Entrez Gene ID: (Human) 1645
3-keto sterol reductase activity
alditol:NADP+ 1-oxidoreductase activity
carbonyl reductase (NADPH) activity
estradiol 17-beta-dehydrogenase activity
protein binding
alcohol dehydrogenase (NADP+) activity
oxidoreductase activity
oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
carboxylic acid binding
bile acid binding
3-beta-hydroxy-5-beta-steroid dehydrogenase (NADP+) activity
steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
17-beta-hydroxysteroid dehydrogenase (NAD+) activity
17-alpha,20-alpha-dihydroxypregn-4-en-3-one dehydrogenase activity
androsterone dehydrogenase activity
5-alpha-androstane-3-beta,17-beta-diol dehydrogenase (NADP+) activity
androstan-3-alpha,17-beta-diol dehydrogenase activity
testosterone 17-beta-dehydrogenase (NADP+) activity
ketosteroid monooxygenase activity
trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity
indanol dehydrogenase activity
17-beta-hydroxysteroid dehydrogenase (NADP+) activity
3-alpha-hydroxysteroid 3-dehydrogenase [NAD(P)+] activity
retinoid metabolic process
prostaglandin metabolic process
xenobiotic metabolic process
digestion
steroid metabolic process
bile acid metabolic process
bile acid and bile salt transport
intestinal cholesterol absorption
epithelial cell differentiation
monocarboxylic acid metabolic process
hormone metabolic process
progesterone metabolic process
retinal metabolic process
cholesterol homeostasis
daunorubicin metabolic process
doxorubicin metabolic process
cellular response to jasmonic acid stimulus
positive regulation of reactive oxygen species metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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