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Invitrogen
APLF Polyclonal Antibody
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FIGURE: 1 / 2
APLF Antibody (PA5-114861) in ICC/IF
Immunocytochemistry analysis of APLF in A549 cells (H2O2 treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with APLF polyclonal antibody (Product # PA5-114861) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-114861
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human APLF(Accession Q8IW19), corresponding to amino acid residues L92-G142.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total APLF.
Target Information
C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks.[supplied by OMIM, Mar 2008].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aprataxin- and PNK-like factor; apurinic-apyrimidinic endonuclease APLF; PNK and APTX-like FHA domain-containing protein; PNK and APTX-like FHA protein; unnamed protein product; XRCC1-interacting protein 1; zinc finger, CX5CX6HX5H motif containing 1
Gene Aliases: 2010301N04Rik; APFL; C2orf13; PALF; RGD1565557; Xip1; ZCCHH1
UniProt ID: (Human) Q8IW19, (Mouse) Q9D842
Entrez Gene ID: (Human) 200558, (Mouse) 72103, (Rat) 500247
nucleotide binding
DNA-(apurinic or apyrimidinic site) lyase activity
endodeoxyribonuclease activity
protein binding
3'-5' exonuclease activity
histone binding
protein binding involved in protein folding
poly-ADP-D-ribose binding
histone chaperone activity
ADP-D-ribose modification-dependent protein binding
single strand break repair
DNA repair
double-strand break repair
double-strand break repair via nonhomologous end joining
protein folding
cellular response to DNA damage stimulus
embryo implantation
regulation of epithelial to mesenchymal transition
regulation of isotype switching
protein localization to chromatin
DNA repair-dependent chromatin remodeling
regulation of DNA repair
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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