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Invitrogen
SLC4A1 Polyclonal Antibody
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FIGURE: 1 / 2
SLC4A1 Antibody (PA5-114893) in ICC/IF
Immunocytochemistry analysis of SLC4A1 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114893) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-114893
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SLC4A1(Accession P02730), corresponding to amino acid residues L333-V383.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Band 3/AE 1.
Target Information
The Band 3 protein is the most abundant integral protein of human erythrocyte membranes and functions as the major anion transporter polypeptide of erythrocytes. Band 3 is a 90-100 kDa protein, its microheterogeneity is ascribed mainly to the structural heterogeneity of the carbohydrate moiety. The C-terminal portion of the molecule spans the membrane several times, and is responsible for anion transport. The N-terminal portion, comprising almost half of the polypeptide chain of Band 3, is located inside the red cell and interacts with cytoskeletal and cytoplasmic proteins such as ankyrin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AE 1; AE1; band 3; anion exchange protein 1; anion exchanger 1; anion exchanger-1; band 3 anion exchanger; CAMP; Diego blood group; erythrocyte membrane protein band 3; erythroid anion exchange protein; Froese blood group; MEB3; MGC116750; MGC116753; MGC126619; MGC126623; solute carrier family 4 (anion exchanger), member 1 (Diego blood group); solute carrier family 4 member 1; solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group); solute carrier family 4, anion exchanger, number 1; Swann blood group; UNIMAS band 3; AE1; unnamed protein product; Waldner blood group; Wright blood group; ZNF828
Gene Aliases: AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR; l11Jus51; RTA1A; SAO; SPH4; SW; WD; WD1; WR
UniProt ID: (Human) P02730, (Mouse) P04919
Entrez Gene ID: (Human) 6521, (Mouse) 20533
inorganic anion exchanger activity
protein binding
cytoskeletal protein binding
anion transmembrane transporter activity
bicarbonate transmembrane transporter activity
chloride transmembrane transporter activity
hemoglobin binding
ankyrin binding
protein homodimerization activity
protein anchor
chloride:bicarbonate antiporter activity
actin binding
ion transmembrane transporter activity
secondary active transmembrane transporter activity
enzyme binding
macromolecular complex binding
anion transport
chloride transport
cellular ion homeostasis
bicarbonate transport
regulation of intracellular pH
transmembrane transport
chloride transmembrane transport
blood coagulation
response to carbon dioxide
response to activity
phospholipid scrambling
negative regulation of urine volume
positive regulation of T cell proliferation
response to hydrogen peroxide
pH elevation
response to arsenic-containing substance
erythrocyte development
negative regulation of oxidoreductase activity
protein localization to plasma membrane
negative regulation of glycolytic process through fructose-6-phosphate
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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